{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:14:29Z","timestamp":1785892469505,"version":"3.56.0"},"reference-count":24,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>This study investigated correlations among ram semen quality parameters, testicular vascularity and echotexture. Measurements were performed biweekly for a year, before (<jats:styled-content style=\"fixed-case\">BE<\/jats:styled-content>) and after ejaculation (<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>), on seven Chios breed rams [three rams (group A) and four rams (group B) of 2\u20136 and 9\u201313\u00a0years old, respectively], considering breeding (1) and non\u2010breeding period (2). Hemodynamics [Pulsatility Index (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>), Resistive Index (<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>)] were evaluated in each testis. Images of testicular parenchyma were analysed to evaluate echogenicity [Mean Value (<jats:styled-content style=\"fixed-case\">MV<\/jats:styled-content>), Contrast (Con), Gray Value Distribution (<jats:styled-content style=\"fixed-case\">GVD<\/jats:styled-content>), Run Length Distribution (RunLD), Long Run Emphasis (LRunEm)]. Semen volume (V), concentration (C), kinetics (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), morphology (Sperm Blue), viability (Eosin\u2010Nigrosin) and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation (Acridine Orange) were assessed. In group A\/2, semen V was correlated to left testis <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> and right testis <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> BE<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.4, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.013\/<jats:italic>r<\/jats:italic>\u00a0=\u00a00.6, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001, respectively), and to right testis <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AE<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.5, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). In A\/1\/AE, semen C was correlated to left testis <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.5, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.003). In group A\/1\/BE, <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content> of both testes was correlated to total and tail abnormalities (left: <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.5, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.008\/ <jats:italic>p<\/jats:italic>\u00a0=\u00a00.004, respectively \u2013 right: <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.4\/<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.5, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.01\/ <jats:italic>p<\/jats:italic>\u00a0=\u00a00.008, respectively). In group B\/1\/BE, sperm tail and total abnormalities were correlated to left testis Con (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.5, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.02\/ <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001, respectively), whereas in A\/2\/BE, they were correlated to left testis LRunEm (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.8\/<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.7, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001, respectively). In group A\/1\/BE, <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage was correlated to right testis LRunEm (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.6, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and tail abnormalities to left testis <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.5, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.03). In group B\/1\/AE, right testis LRunEm, <jats:styled-content style=\"fixed-case\">GVD<\/jats:styled-content>, RunLD, were correlated to sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> integrity (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.9, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Immotile spermatozoa and viability were correlated to left testis Con in group B\/2\/AE (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.6\/<jats:italic>r<\/jats:italic>\u00a0=\u00a00.6, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) and to right testis <jats:styled-content style=\"fixed-case\">MV<\/jats:styled-content> in group A\/1\/AE (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.6\/<jats:italic>r<\/jats:italic>\u00a0=\u00a00.6, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). In conclusion, alterations in both ram testes blood flow and echotexture seem to be moderately correlated with sperm assessed parameters, depending on season and ram age.<\/jats:p>","DOI":"10.1111\/rda.13279","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T22:33:05Z","timestamp":1537569185000},"page":"50-55","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Effects of testicular hemodynamic and echogenicity changes on ram semen characteristics"],"prefix":"10.1111","volume":"53","author":[{"given":"Aikaterini","family":"Ntemka","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelos","family":"Kiossis","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantin","family":"Boscos","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandros","family":"Theodoridis","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Kourousekos","sequence":"additional","affiliation":[{"name":"Directorate of Veterinary Centre of Thessaloniki Department of Reproduction and Artificial Insemination National Ministry of Rural Development and Food  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Tsakmakidis","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-007-9010-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2011.011050"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.10.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcu.20439"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2318-3659.v50i5p384-395"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781845934507.0000"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.07.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01259-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JARG.0000031251.57848.04"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01303.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.05.019"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2006.10.003"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136725"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.09.007"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.066"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2003.00436.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.10.001"},{"key":"e_1_2_8_19_1","unstructured":"Scheibenzuber E. 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