{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T05:56:15Z","timestamp":1778392575675,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T00:00:00Z","timestamp":1534896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR\/10247\/AAQ\/1\/364\/2007"],"award-info":[{"award-number":["BT\/PR\/10247\/AAQ\/1\/364\/2007"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>In view of the silent nature of estrus in buffalo, a noninvasive assay kit has long been felt necessary for easy and effective estrus detection. This study was designed to detect estrus in buffalo using a kit formulated in our laboratory based on pheromone compound. Group I: Urine samples collected at estrus phase and group II: randomly collected urine samples were subjected to the test using the kit. No colour developed (i.e., positive reaction) in estrus urine after adding the kit solution. By contrast, pale and\/or dark pink colour developed (i.e., negative reaction) in urine from the proestrus and diestrus buffaloes, respectively. Field evaluation of the kit in groups I and II revealed that 60.87% and 71.43% of urine samples were correctly identified as estrus and nonestrus (i.e., proestrus and diestrus), respectively. Therefore, the first of its kind estrus detection kit formulated based on urinary pheromone can as well be used as a simple device to detect estrus in buffalo.<\/jats:p>","DOI":"10.1111\/rda.13281","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T09:36:58Z","timestamp":1532511418000},"page":"1466-1472","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of pheromone\u2010based kit: A noninvasive approach of estrus detection in buffalo"],"prefix":"10.1111","volume":"53","author":[{"given":"Subramanian","family":"Muthukumar","sequence":"first","affiliation":[{"name":"Center for Pheromone Technology, Department of Animal Science Bharathidasan University Tiruchirappalli Tamil Nadu India"},{"name":"Center for Animal Research, Training and Services (CAReTS), Central Inter\u2010Disciplinary Research Facility (CIDRF) Mahatma Gandhi Medical College and Research Institute (MGMC\u2010RI) Campus Puducherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuthirapandi","family":"Muniasamy","sequence":"additional","affiliation":[{"name":"Center for Pheromone Technology, Department of Animal Science Bharathidasan University Tiruchirappalli Tamil Nadu India"},{"name":"Department of Microbiology Ayya Nadar Janaki Ammal College (Autonomous)  Sivakasi Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahalingam","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Center for Pheromone Technology, Department of Animal Science Bharathidasan University Tiruchirappalli Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andivelu","family":"Ilangovan","sequence":"additional","affiliation":[{"name":"School of Chemistry Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soundarapandian","family":"Satheshkumar","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology Madras Veterinary College  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thangavel","family":"Rajagopal","sequence":"additional","affiliation":[{"name":"Post Graduate and Research Department of Zoology and Microbiology Thiagarajar College (Autonomous)  Madurai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Velusamy","family":"Ramesh Saravana Kumar","sequence":"additional","affiliation":[{"name":"Department of Livestock Production and Management Veterinary College and Research Institute  Namakkal Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuppusamy","family":"Sivakumar","sequence":"additional","affiliation":[{"name":"Department of Livestock Production and Management Veterinary College and Research Institute  Namakkal Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7094-0351","authenticated-orcid":false,"given":"Govindaraju","family":"Archunan","sequence":"additional","affiliation":[{"name":"Center for Pheromone Technology, Department of Animal Science Bharathidasan University Tiruchirappalli Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1958.172322x"},{"key":"e_1_2_9_3_1","first-page":"227","article-title":"Vertebrate Pheromone and their biological importance","volume":"12","author":"Archunan G.","year":"2009","journal-title":"Journal of Experimental Zoology, India"},{"key":"e_1_2_9_4_1","unstructured":"Archunan G. &Muthukumar S.(2015).Methods to identify the proper time of insemination in buffalo based on urinary pheromone compound. 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