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A total of 23 cyclic ewes received two doses of 37.5\u00a0\u03bcg of d\u2010cloprostenol by latero\u2010vulvar route 7\u00a0days apart. After the second injection of d\u2010cloprostenol, the ewes were checked for oestrus (every 12\u00a0hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5\u00a0\u03bcg of d\u2010cloprostenol (latero\u2010vulvar) and 1\u00a0mg of oestradiol benzoate by either intramuscular (EBim group; <jats:italic>n<\/jats:italic>\u00a0=\u00a012) or intravaginal (EBivg group; <jats:italic>n<\/jats:italic>\u00a0=\u00a011) route 16\u00a0hr before embryo flushing. Twenty minutes before the flushing, 50\u00a0IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d\u2010cloprostenol injection) was 91.3% (21\/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7\u00a0\u00b1\u00a02.0\u00a0min compared 26.2\u00a0\u00b1\u00a01.9\u00a0min), the flushing fluid recovery rate (94.8\u00a0\u00b1\u00a01.3% compared with 91.0\u00a0\u00b1\u00a02.9%) and the average number of structures recovered per ewe (0.5\u00a0\u00b1\u00a00.4 compared with 0.8\u00a0\u00b1\u00a00.4) did not vary (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7\/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous\u2010synchronized Santa In\u00eas ewes pretreated with d\u2010cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.<\/jats:p>","DOI":"10.1111\/rda.13318","type":"journal-article","created":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T07:38:21Z","timestamp":1535787501000},"page":"118-125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Combined treatment with oestradiol benzoate, d\u2010cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-3984","authenticated-orcid":false,"given":"Jeferson Ferreira","family":"da Fonseca","sequence":"first","affiliation":[{"name":"Embrapa Caprinos e Ovinos Sobral Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana Nunes","family":"Zambrini","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Domingos","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcio Roberto","family":"Silva","sequence":"additional","affiliation":[{"name":"Embrapa Gado de Leite Juiz de Fora Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-290X","authenticated-orcid":false,"given":"Maria Emilia Franco","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Zandonadi","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel Mieczyslaw","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna Maria Gon\u00e7alves","family":"Souza\u2010Fabjan","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(90)90614-Y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.02.024"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874255601103010162"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-35982012000600014"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00278"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00268-X"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-4162-10196"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-41626783"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2017.10.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.025"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2012.08.007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90121-X"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352009000200005"},{"key":"e_1_2_8_16_1","first-page":"1","article-title":"Colheita transcervical de ovinos da ra\u00e7a Santa In\u00eas no semi\u2010\u00e1rido nordestino","volume":"8","author":"Gusm\u00e3o A. 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