{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T06:45:40Z","timestamp":1775976340931,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2018,11,25]],"date-time":"2018-11-25T00:00:00Z","timestamp":1543104000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Aim of the study was to examine the effect of deslorelin on uterine tissues of eleven pre\u2010pubertal bitches aged 4.2\u00a0\u00b1\u00a00.6\u00a0m. Implants containing placebo (sodium chloride 0.9%; <jats:italic>n<\/jats:italic>\u00a0=\u00a04, G I), 4.7\u00a0mg (<jats:italic>n<\/jats:italic>\u00a0=\u00a03, <jats:styled-content style=\"fixed-case\">GII<\/jats:styled-content>) or 9.4\u00a0mg (<jats:italic>n<\/jats:italic>\u00a0=\u00a04, <jats:styled-content style=\"fixed-case\">GIII<\/jats:styled-content>) deslorelin acetate (Suprelorin<jats:sup>\u00ae<\/jats:sup>; Virbac, France), were administered subcutaneously. Signs of oestrus, vaginal cytology, serum progesterone (P4) and estradiol\u201017\u03b2 (E2) concentrations were monitored until the occurrence of oestrus. Bitches were ovariohysterectomized and sections from the uterine tissue were subjected to immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) for detection of Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> receptor (R), Kisspeptin (<jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>)10, Kisspeptin receptor (<jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54), androgen receptor (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>), oestrogen receptor (<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>) \u03b1,\u03b2, and progesterone receptor (<jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content>). Tissue sections were scored semi\u2010quantitatively using an immunoreactivity score (<jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content>) ranging from 0 to 300 (3). Since some animals were ovariohysterectomized before puberty (<jats:italic>n<\/jats:italic>\u00a0=\u00a01 from <jats:styled-content style=\"fixed-case\">GII<\/jats:styled-content> and <jats:italic>n<\/jats:italic>\u00a0=\u00a02 from <jats:styled-content style=\"fixed-case\">GIII<\/jats:styled-content>), and some in metestrus (all controls and 2 from <jats:styled-content style=\"fixed-case\">GII<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GIII<\/jats:styled-content> each), results from these animals were separately evaluated and compared to the controls. Results: No abnormalities were seen in uterine tissues. Kisspeptin 10 expression was low in all cell types, highest <jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content> were seen in the vascular endothelial cells. The <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 was mainly detected in the luminal epithelial cells, superficial and deep uterine glands. The expression of <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 and <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u03b1,\u03b2 was especially high in bitches operated prepubertally. No difference was observed between the controls and experimental bitches operated in their first metestrus. The <jats:styled-content style=\"fixed-case\">PR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u03b1,\u03b2 were exclusively expressed in superficial and deep uterine glands and luminal surface epithelial cells. The <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> and Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010R expression was negative in all cells of all groups. We conclude that application of 4.7 or 9.4\u00a0mg deslorelin at the age of 4\u00a0months did not cause uterine disturbances. <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 expression might be influenced by pre\u2010pubertal deslorelin treatment or the changings related to approaching puberty; the latter is supposed in case of <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u03b1,\u03b2.<\/jats:p>","DOI":"10.1111\/rda.13337","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T06:07:31Z","timestamp":1543212451000},"page":"103-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Pre\u2010pubertal treatment with a Gn<scp>RH<\/scp> agonist in bitches\u2014Effect on the uterus and hormone receptor expression"],"prefix":"10.1111","volume":"53","author":[{"given":"Sabine","family":"Sch\u00e4fer\u2010Somi","sequence":"first","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer Vetmeduni Vienna  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duygu","family":"Kaya","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Obstetrics and Gynecology University of Kafkas  Kars Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmut","family":"S\u00f6zmen","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Pathology Ondokuz Mayis University  Samsun Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Semra","family":"Kaya","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Obstetrics and Gynecology University of Kafkas  Kars Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selim","family":"Aslan","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Department of Obstetrics and Gynecology Near East University  Nicosia Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.01.019"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.028779"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12616"},{"key":"e_1_2_9_5_1","first-page":"3","article-title":"Biology of gonadotrophin secretion in adult and prepubertal female dogs","volume":"47","author":"Concannon P. 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