{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:13:42Z","timestamp":1788930822114,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,13]],"date-time":"2018-10-13T00:00:00Z","timestamp":1539388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Two experiments were carried out to determine the efficiency of supplementation of ram semen extender with caffeine on chilled storage and frozen capacity of spermatozoa. In the first experiment, eighty ejaculates were collected by an artificial vagina from five adult Barki rams, aged 2\u20133\u00a0years and weighted 45.0\u00a0\u00b1\u00a02.0\u00a0kg throughout the experimental period (January to February 2017). The ejaculates were pooled and diluted (1:10) with tris\u2010citric egg yolk extender and were split into five groups. Group 1 served as control, whereas groups 2\u20105 were supplemented with 0.1, 0.2, 0.3 and 0.4\u00a0<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content> caffeine. All diluted semen specimens were evaluated for physical characteristics immediately after dilution (T<jats:sub>0<\/jats:sub>) and throughout preservation period of 48\u00a0hr at 4\u00b0C. Simultaneously, oxidative stress and indices such as total antioxidant capacity (<jats:styled-content style=\"fixed-case\">TAC<\/jats:styled-content>), malondialdehyde concentrations (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>) and alkaline transaminase (<jats:styled-content style=\"fixed-case\">AKP<\/jats:styled-content>) concentrations and value of resazurin reduction test (<jats:styled-content style=\"fixed-case\">RRT<\/jats:styled-content>) were determined. In the second experiment, the raw pooled ejaculates were diluted (1:10) with glycerolated tris\u2010citric egg yolk extender, receiving the previously mentioned caffeine levels. The post\u2010thaw assessment of cryopreserved spermatozoa, in all groups, was conducted by a computer\u2010assisted sperm analysis (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>) system. The results revealed that adding caffeine to ram semen extender at low (0.1\u00a0<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>) or medium (0.2\u00a0<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>) levels had positive impact on both physical characteristics of ram sperm and the enzymatic activities compared to the other semen groups. Caffeine supplementation also enhanced post\u2010thaw sperm dynamics, which implies its potential as an exogenous antioxidant supplement.<\/jats:p>","DOI":"10.1111\/rda.13339","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T16:07:41Z","timestamp":1536682061000},"page":"225-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-3004","authenticated-orcid":false,"given":"Ibrahim S.","family":"Abd El\u2010Hamid","sequence":"first","affiliation":[{"name":"Animal &amp; Poultry Production Division Desert Research Center Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1992.tb02609.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080420311"},{"issue":"77","key":"e_1_2_9_4_1","first-page":"607","article-title":"Effects of amphora coffeaeformis algae extract on morphology and antioxidant enzymes of cryopreserved buffalo spermatozoa","volume":"3","author":"Badr M. 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