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Samples were collected from testes, epididymides, seminal vesicles and bulbourethral glands of four rams. Expression of clusterin (CLU), osteopontin (OPN) and prostaglandin D2 synthase (PGDS) genes were evaluated in all samples by real\u2010time PCR. Shotgun proteomic analysis was performed using samples from the head, corpus and cauda epididymides and from all other structures as well. Gene ontology terms and protein interactions were obtained from UniProtKB databases and MetaCore v.6.8 platform. CLU trasncripts were detected in the testes, epididymides, seminal vesicles and bulbourethral glands of the Somalis rams. The initial region and body of the epididymis had the greatest CLU expression. OPN mRNA was localized in all tissues of the ram reproductive tract. PGDS mRNA was detected in the testes and epididymides. Lable\u2010free mass spectrometry allowed the identification of 137 proteins in all samples. Proteins of the epididymis head mainly participate in cellular processes and response to stimulus, participating in catalityc activity and binding. Proteins of epididymis body acted as regulatory proteins and in cellular processes, with binding and catalytic activity. Cauda epididymis molecules were associated with cellular processes and regulation, with binding function and catalytic activity as well. Testis proteins were mainly linked to cell processes and response to stimuli, and had catalytic function. Seminal vesicle proteins were involved in regulation and mainly with binding functions. Most bulbourethral gland proteins participated in cellular processes. The present study is the first to evaluate the proteome and gene expressions in the reproductive tract of Brazilian Somalis rams. Such pieces of information bring significant cointribution for the understanding of the reproductive physiology of locally\u2010adapted livestock.<\/jats:p>","DOI":"10.1111\/rda.13348","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T08:10:45Z","timestamp":1537776645000},"page":"939-948","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gene and protein expression in the reproductive tract of Brazilian Somalis rams"],"prefix":"10.1111","volume":"54","author":[{"given":"Maria J\u00falia B.","family":"Bezerra","sequence":"first","affiliation":[{"name":"Department of Animal Science Federal University of Ceara Fortaleza, Ceara Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Animal Science Federal University of Ceara Fortaleza, Ceara 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