{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:47:20Z","timestamp":1790876840399,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T00:00:00Z","timestamp":1545955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>In horse breeding, quality assessment of semen before insemination is often requested. Non\u2010laboratory\u2010based techniques for objective analysis of sperm motility are thus of interest. The aim of this study was evaluating a portable device for semen analysis (Ongo sperm test) and its comparison with computer\u2010assisted semen analysis (CASA). Semen was collected from 10 stallions, diluted to 100, 50 and 25\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml and analysed for total (TM) and progressive motility (PM). The final sperm concentration influenced total motility analysed by Ongo (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) which was higher at 100\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml when compared to 25\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) but not when compared to 50\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml (n.s.). Sperm concentration did not influence total motility when assessed by SpermVision (n.s.). Agreement between methods was evaluated by correlation analysis and Bland\u2013Altman plot. Intra\u2010assay variation of Ongo was 5.2%\u00a0\u00b1\u00a03.0 for TM and 6.9%\u00a0\u00b1\u00a03.4 for PM. Correlation between Ongo and CASA was <jats:italic>r<\/jats:italic>\u00a0=\u00a00.79, 0.88 and 0.83 for 100, 50 and 25\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml for TM, and <jats:italic>r<\/jats:italic>\u00a0=\u00a00.87, 0.89 and 0.87 for PM, respectively (all <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). At the 100 and 25\u00a0mio\/ml dilutions, the difference between the two systems deviated significantly from 0, while no such bias existed at the 50\u00a0mio\/ml dilution (TM Ongo 85.0%, CASA 82.3%; PM Ongo 64.1%, CASA 66.1%). The 95% confidence interval was 19.9%, 18.9% and 19.2%\u00a0\u00b1\u00a0mean for TM and 20.7%, 17.4% and 20.3%\u00a0\u00b1\u00a0mean for 100, 50 and 25\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml, respectively. In conclusion, Ongo sperm test sperm motility data were strongly correlated with data obtained by CASA. In addition, at a concentration of 50\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml values measured with both systems were close to identical. At this concentration, which is recommended in equine AI, Ongo and CASA can be used interchangeably.<\/jats:p>","DOI":"10.1111\/rda.13390","type":"journal-article","created":{"date-parts":[[2018,12,28]],"date-time":"2018-12-28T11:21:37Z","timestamp":1545996097000},"page":"514-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of a portable device for assessment of motility in stallion semen"],"prefix":"10.1111","volume":"54","author":[{"given":"Tammo","family":"Buss","sequence":"first","affiliation":[{"name":"Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses Vetmeduni Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8849-9189","authenticated-orcid":false,"given":"J\u00f6rg","family":"Aurich","sequence":"additional","affiliation":[{"name":"Section for Obstetrics, Gynecology and Andrology, Department 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