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Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation\u2010like status of the acrosome may benefit probability of pregnancy in cows.<\/jats:p>","DOI":"10.1111\/rda.13401","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T07:06:17Z","timestamp":1546931177000},"page":"604-612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed\u2010time artificial insemination"],"prefix":"10.1111","volume":"54","author":[{"given":"Denise S.","family":"Okano","sequence":"first","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8592-4020","authenticated-orcid":false,"given":"Jurandy M.","family":"Penitente\u2010Filho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor E.","family":"Gomez Le\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6677-9609","authenticated-orcid":false,"given":"Paula P.","family":"Maitan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila O.","family":"Silveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"Waddington","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar A.","family":"D\u00edaz\u2010Miranda","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo P.","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone E. 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