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In the present study, we validated a protocol for the extraction of RNA from FFPE samples collected from healthy and diseased equine placenta. The quality and quantity of the extracted RNA from the FFPE and matching RNA<jats:italic>later<\/jats:italic>\u2122\u2010preserved samples and expression levels of common housekeeping genes and reference microRNAs were evaluated. Precision of the expression data was evaluated by comparing relative expression of CYP19A1 and HSD3B1 in FFPE and RNA<jats:italic>later<\/jats:italic>\u2122 samples. The median RNA concentration recovered from FFPE samples was 316.8\u00a0ng\/mm<jats:sup>3<\/jats:sup> of tissue (ranging between 61.6 and 917.4\u00a0ng\/mm<jats:sup>3<\/jats:sup>), average RNA integrity number was 2.3\u00a0\u00b1\u00a00.9 (mean\u00a0\u00b1\u00a0standard deviation), and 84% of samples had RNA fragments longer than 200 nucleotides (DV<jats:sub>200<\/jats:sub>). RNA concentrations and <jats:italic>C<\/jats:italic><jats:sub>T<\/jats:sub> values for GAPDH, ACTB, miR\u20108908a and miR\u2010369 in FFPE samples were significantly correlated (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u22120.8, \u22120.7, \u22120.4 and \u22120.4, respectively; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Expression pattern of normalized CYP19A1 and HSD3B1 in paired FFPE and RNA<jats:italic>later<\/jats:italic>\u2122 samples was significantly correlated (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.97 for CYP19A1 and HSD3B1; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). This study demonstrates that RNA can be extracted from FFPE equine placental tissue and used for downstream transcriptomic analysis. Similar RNA expression patterns were obtained using RNA<jats:italic>later<\/jats:italic>\u2122 and FFPE tissue samples.<\/jats:p>","DOI":"10.1111\/rda.13406","type":"journal-article","created":{"date-parts":[[2019,1,19]],"date-time":"2019-01-19T20:14:12Z","timestamp":1547928852000},"page":"627-634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Extraction of RNA from formalin\u2010fixed, paraffin\u2010embedded equine placenta"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5553-5967","authenticated-orcid":false,"given":"Pouya","family":"Dini","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"},{"name":"Department of Veterinary Science University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Esteller\u2010Vico","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsten E.","family":"Scoggin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7884-0307","authenticated-orcid":false,"given":"Peter","family":"Daels","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0502-0276","authenticated-orcid":false,"given":"Barry A.","family":"Ball","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science University of Kentucky Lexington Kentucky"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-1578(10)60449-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6728-5_5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/448959a"},{"key":"e_1_2_7_5_1","first-page":"48","article-title":"Alpha\u2010fetoprotein is present in the fetal fluids and is increased in plasma of mares with experimentally induced ascending placentitis","volume":"154","author":"Canisso I. 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