{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T08:19:48Z","timestamp":1776154788358,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,18]],"date-time":"2019-05-18T00:00:00Z","timestamp":1558137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007613","name":"Ankara Universitesi","doi-asserted-by":"publisher","award":["15B0239006"],"award-info":[{"award-number":["15B0239006"]}],"id":[{"id":"10.13039\/100007613","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to determine the serum concentrations, ovarian presence and expression of anti\u2010M\u00fcllerian hormone (AMH) in pre\u2010pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and\/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre\u2010pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre\u2010pubertal groups, this increase was statistically non\u2010significant (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.<\/jats:p>","DOI":"10.1111\/rda.13451","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T13:46:26Z","timestamp":1556891186000},"page":"979-986","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immunohistochemical and qPCR determination of the expression and serum level of anti\u2010M\u00fcllerian hormone in pre\u2010pubertal, intact and ovarian remnant syndrome detected bitches"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9177-9299","authenticated-orcid":false,"given":"Kubra","family":"Karakas Alkan","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Ceylan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8332-5334","authenticated-orcid":false,"given":"Hasan","family":"Alkan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Selcuk University Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dogukan","family":"Ozen","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alev Gurol","family":"Bayraktaroglu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Kaymaz","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Ankara University Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.03.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.11.016"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.11.7588229"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0800509"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00714.x"},{"key":"e_1_2_9_7_1","first-page":"1214","article-title":"Current and proposed methods for contraception and termination of pregnancy in dogs and cats","volume":"198","author":"Concannon P. 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