{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:00:42Z","timestamp":1787238042362,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,9]]},"abstract":"Abstract<\/jats:title>\n \n Inflammation and fibroproliferative diseases may be modulated by epigenetic changes. Therefore, we suggest that epigenetic mechanisms could be involved in equine endometrosis pathogenesis. DNA methylation is one of the methods to evaluate epigenetics, through the transcription of methyltransferases (\n DNMT1, DNMT3A, DNMT3B<\/jats:italic>\n ). The correlation between\n DNMTs<\/jats:italic>\n and collagen\n (COL<\/jats:italic>\n ) transcripts was assessed for the different Kenney and Doig's (\n Current Therapy in Theriogenology<\/jats:italic>\n . Philadelphia: WB Saunders; 1986) endometrium categories. Endometrial biopsies were randomly collected from cyclic mares. Histological classification (category I,\n n\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 13; II A,\n n\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 17; II B,\n n\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 12; and III,\n n\u00a0<\/jats:italic>\n =\n \u00a0<\/jats:italic>\n 7) and evaluation of\n COL1A2, COL3A1<\/jats:italic>\n and\n DNMTs<\/jats:italic>\n transcripts by qPCR, were performed. Data were analysed by one\u2010way analysis of variance (ANOVA), Kruskal\u2013Wallis test and Pearson correlation. As mares aged, there was an increase in endometrium fibrosis (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .01), and in\n DNMT1<\/jats:italic>\n mRNA (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .001). Considering\n DNMT3B<\/jats:italic>\n transcripts for each category, there was an increase with fibrosis (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .05). No changes were observed for\n DNMT1<\/jats:italic>\n and\n DNMT3A<\/jats:italic>\n transcripts. However,\n DNMT3A<\/jats:italic>\n mRNA levels were the highest in all categories (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .01). In category I endometrium, a positive correlation was observed for transcripts of all\n DNMTs<\/jats:italic>\n in both\n COLs<\/jats:italic>\n (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .01). In category IIA, this correlation was also maintained for all\n DNMTs<\/jats:italic>\n transcripts in\n COL1A2<\/jats:italic>\n (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .05), but only for\n DNMT3B<\/jats:italic>\n in\n COL3A1<\/jats:italic>\n (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .05). In category IIB, there was a positive correlation between\n DNMT3B<\/jats:italic>\n and\n COL3A1<\/jats:italic>\n (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .05). In category III, a positive correlation was only observed between\n DNMT3B<\/jats:italic>\n and\n COL3A1<\/jats:italic>\n (\n p\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n .05). Our results suggest that there is a disturbance in\n COLs<\/jats:italic>\n and\n DNMTs<\/jats:italic>\n correlation during fibrosis.\n <\/jats:p>","DOI":"10.1111\/rda.13515","type":"journal-article","created":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T01:56:37Z","timestamp":1568253397000},"page":"46-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Collagens and DNA methyltransferases in mare endometrosis"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5499-4563","authenticated-orcid":false,"given":"Joana","family":"Alpoim\u2010Moreira","sequence":"first","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosa","family":"Rebord\u00e3o","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal"},{"name":"Coimbra College of Agriculture Polytechnic Institute of Coimbra Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0075-262X","authenticated-orcid":false,"given":"Ana","family":"Amaral","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Pinto\u2010Bravo","sequence":"additional","affiliation":[{"name":"Coimbra College of Agriculture Polytechnic Institute of Coimbra Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Bliebernicht","sequence":"additional","affiliation":[{"name":"Embriovet Muge Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dariusz J.","family":"Skarzynski","sequence":"additional","affiliation":[{"name":"Polish Academy of Science Olsztyn Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gra\u00e7a","family":"Ferreira\u2010Dias","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health Faculty of Veterinary Medicine University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201205-0888OC"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2144\/04371RR03"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm4043"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-1957"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090999"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-31390-7_7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2012.1324"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2009.55"},{"key":"e_1_2_8_10_1","unstructured":"Kenney R.M.(1992).The aetiology diagnosis and classification of chronic degenerative endometritis. In: Proceedings of the John P. Hughes International Workshop on Equine endometritis. Newmarket UK.Equine Veterinary Journal 25 186."},{"key":"e_1_2_8_11_1","first-page":"723","volume-title":"Current Therapy in Theriogenology","author":"Kenney R. 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