{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:58Z","timestamp":1787015998049,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:00:00Z","timestamp":1565049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372280"],"award-info":[{"award-number":["31372280"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Granulosa cells play important roles in the regulation of ovarian functions. Phospholipase C is crucial in several signalling pathways and could participate in the molecular mechanisms of cell proliferation, differentiation and ageing. The objective of this study was to identify the effects of phospholipase C on the steroidogenesis of oestradiol and progesterone in porcine granulosa cells cultured in vitro. Inhibitor U73122 or activator m\u20103M3FBS of phospholipase C was added to the in vitro medium of porcine granulosa cells, respectively. The secretion of oestradiol decreased after 2\u00a0hr, 8\u00a0hr, 12\u00a0hr, 24\u00a0hr and 48\u00a0hr of treatment with 500\u00a0nM U73122 (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) and decreased after 2\u00a0hr of treatment in the 500\u00a0nM m\u20103M3FBS addition group (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). The secretion of progesterone increased after 4\u00a0hr of treatment with 500\u00a0nM U73122 (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) and increased after 2\u00a0hr and 8\u00a0hr of treatment in the 500\u00a0nM m\u20103M3FBS addition group (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). The ratio of oestradiol to progesterone decreased at each time point, except 8\u00a0hr after the addition of 500\u00a0nM U73122 (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). The ratio of oestradiol to progesterone decreased after 2\u00a0hr (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) of treatment with 500\u00a0nM m\u20103M3FBS. In genes that regulate the synthesis of oestradiol or progesterone, the mRNA expression of\n                    <jats:italic>CYP11A1<\/jats:italic>\n                    was markedly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05), and the mRNA expression of other genes did not change significantly in the U73122 treatment group, while the addition of m\u20103M3FBS did not change those genes significantly despite the contrary trend. Our results demonstrated that phospholipase C can be a potential target to stimulate the secretion of oestradiol and suppress progesterone secretion in porcine granulosa cells cultured in vitro, which shed light on a novel biological function of phospholipase C in porcine granulosa cells.\n                  <\/jats:p>","DOI":"10.1111\/rda.13517","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T14:04:12Z","timestamp":1563458652000},"page":"1236-1243","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The effects of phospholipase C on oestradiol and progesterone secretion in porcine granulosa cells cultured in vitro"],"prefix":"10.1111","volume":"54","author":[{"given":"Huali","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling 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