{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:40:30Z","timestamp":1790185230524,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of this study was to develop a non\u2010invasive procedure to stimulate ejaculation in median and small lizards for semen collection. After semen collection, we applied a battery of tests to assess the motility (wave and progressive), viability (eosin\u2013nigrosin stain), morphology (normal\/abnormal), sperm concentration and ejaculate number, seminal volume and colour. We obtained this fluid from all males of the four species of sceloporine lizards (<jats:italic>n<\/jats:italic>\u00a0=\u00a030) and one species of Mexican horned lizards (<jats:italic>n<\/jats:italic>\u00a0=\u00a07). We found that semen from all males had a liquid\u2010like consistency and a milky\u2010white appearance. Ejaculate volume and sperm concentration varied among the males and the species studied. We also observed that although the mobility, viability and normal sperm morphology showed a wide variability, their average value in each species was high. We conclude that \u201cgenital papilla pressure\u201d was an efficient and non\u2010invasive semen obtaining method for small\u2010 and medium\u2010sized lizards that does not imply damage to donors and could be used in other lizard species.<\/jats:p>","DOI":"10.1111\/rda.13536","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T00:37:36Z","timestamp":1571359056000},"page":"54-58","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A non\u2010invasive method for semen collection and evaluation in small and median size lizards"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5766-3599","authenticated-orcid":false,"given":"Mart\u00edn","family":"Mart\u00ednez\u2010Torres","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9423-5278","authenticated-orcid":false,"given":"Uriel \u00c1ngel","family":"S\u00e1nchez\u2010Rivera","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7202-9628","authenticated-orcid":false,"given":"Norma Berenice","family":"Cruz\u2010Cano","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6454-9452","authenticated-orcid":false,"given":"Yab\u00edn Josu\u00e9","family":"Castro\u2010Camacho","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6490-8825","authenticated-orcid":false,"given":"Juana","family":"Luis","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n Facultad de Estudios Superiores Iztacala Universidad Nacional Aut\u00f3noma de M\u00e9xico Tlalnepantla M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2265-6890","authenticated-orcid":false,"given":"Alfredo","family":"Medrano","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n Animal Facultad de Estudios Superiores Cuautitl\u00e1n Universidad Nacional Aut\u00f3noma de M\u00e9xico Cuautitl\u00e1n Izcalli M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,17]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"65","volume-title":"Evaluaci\u00f3n de la criosupervivencia y la fluidez de membrana de espermatozoides de perro enfriados a \u22125\u00b0C, previo a la congelaci\u00f3n","author":"Alcantar R. 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L.","year":"2015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD02050"},{"key":"e_1_2_9_14_1","first-page":"287","volume-title":"WHO laboratory manual for the examination and 20 processing of human semen","author":"World Health Organization","year":"2010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.08.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.20126"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/conphys\/cox062"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.74.12.1536"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.13536","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T16:59:49Z","timestamp":1694192389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":17,"journal-issue":{"issue":"S4","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/rda.13536"],"URL":"https:\/\/doi.org\/10.1111\/rda.13536","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"2019-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}