{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:11Z","timestamp":1787016011054,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Germ cell (GC) transplantation (GCT) is a proven powerful reproductive technique to enhance the production efficiency of domesticated animals and aid to the recovery of endangered germ lines. In mammals, several methods have been adopted for the eradication of GCs such as treatment with cytotoxic drugs, irradiation, cold ischaemia and hyperthermic treatment. Some of these methods have also been tried in fishes, and conditions for sterilization of gonads have been established. Here, we report the production of GC\u2010depleted male striped catfish\n                    <jats:italic>Pangasianodon hypophthalmus<\/jats:italic>\n                    in 12\u00a0weeks by the combination of heat and chemical treatments. The cytotoxic drug busulphan (40\u00a0mg\/kg) was intraperitoneally injected into the animals at 2\u2010week intervals (six doses in total) and maintained in water at 38\u00b0C between weeks 1 and 12. The effectiveness of the treatment was assessed using gonadal index and histology. At the end of 12\u00a0weeks, very severe gonadal degeneration was observed in fish treated with the heat\u2013chemical combination, and 100% of sampled fish (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05) were found devoid of endogenous GCs. On contrary, high temperature alone caused minor gonadal degeneration. Results obtained in this study suggest that endogenous GCs of large\u2010bodied fish such as\n                    <jats:italic>P.\u00a0hypophthalmus<\/jats:italic>\n                    can also be sterilized by heat and chemical treatments within a considerably short period.\n                  <\/jats:p>","DOI":"10.1111\/rda.13564","type":"journal-article","created":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T03:08:01Z","timestamp":1567912081000},"page":"1560-1566","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Depletion of endogenous germ cells in striped catfish\n                    <i>Pangasianodon hypophthalmus<\/i>\n                    (Sauvage, 1878) by heat\u2013chemical treatments"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9389-7467","authenticated-orcid":false,"given":"Sullip Kumar","family":"Majhi","sequence":"first","affiliation":[{"name":"ICAR\u2010National Bureau of Fish Genetic Resources Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradip Kumar","family":"Maurya","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Fish Genetic Resources Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santosh","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Fish Genetic Resources Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vindhya","family":"Mohindra","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Fish Genetic Resources Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuldeep Kumar","family":"Lal","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Fish Genetic Resources Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0044-8486(01)00588-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-6480(82)90035-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.016519"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071607"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11303"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.24.11298"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1577\/A05-008.1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.06.004"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2011.09.010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0143"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:FISH.0000030613.33121.95"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20577"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010740"},{"key":"e_1_2_9_15_1","first-page":"146","article-title":"Germ cells transplantation in fish: The nile tilapia model","volume":"2","author":"Lacerda S. 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P.","year":"1988","journal-title":"Endocrinology"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13564","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13564","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T14:46:18Z","timestamp":1694184378000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,26]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/rda.13564"],"URL":"https:\/\/doi.org\/10.1111\/rda.13564","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,26]]},"assertion":[{"value":"2019-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}