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Melatonin can be metabolized by free radical interaction, but also pseudo\u2010enzymatically or\u00a0by enzymatic pathways. We have previously detected the existence of melatonin\u2010synthesizing enzymes and melatonin receptors MT1 and MT2 in the ram reproductive tract; thus, in order to start to elucidate melatonin catabolism in these organs, we have investigated the presence of the melatonin\u2010catabolizing enzymes indoleamine 2,3\u2010dioxygenase (IDO, both IDO1 and IDO2 isoforms) and myeloperoxidase (MPO) in testis, epididymis and accessory glands. Gene expression analyses by real\u2010time PCR showed the presence of MPO, IDO1 and IDO2 in all the organs of the ram reproductive tract and revealed that MPO is the main melatonin\u2010catabolizing enzyme, which is mainly expressed in the testis and the bulbourethral glands (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). These results were further corroborated by immunohistochemical staining, and by Western blot. Likewise, MPO was also evidenced in epididymal and ejaculated spermatozoa by indirect immunofluorescence and Western blot. In conclusion, melatonin\u2010catabolizing enzymes MPO, IDO1 and IDO2 are expressed in the ram reproductive tract, and MPO is the most expressed one, mainly in the testis and the bulbourethral glands. The presented results warrant further studies on the function of these enzymes and their melatonin\u2010metabolizing activity.<\/jats:p>","DOI":"10.1111\/rda.13574","type":"journal-article","created":{"date-parts":[[2019,10,6]],"date-time":"2019-10-06T08:48:23Z","timestamp":1570351703000},"page":"1643-1650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Presence of melatonin\u2010catabolizing non\u2010specific enzymes myeloperoxidase and indoleamine 2,3\u2010dioxygenase in the ram reproductive tract"],"prefix":"10.1111","volume":"54","author":[{"given":"Paula","family":"Mart\u00ednez\u2010Marcos","sequence":"first","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3929-5064","authenticated-orcid":false,"given":"Melissa","family":"Carvajal\u2010Serna","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"},{"name":"Departamento de Producci\u00f3n Animal Facultad de Medicina Veterinaria y de Zootecnia Universidad Nacional de Colombia Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sof\u00eda","family":"L\u00e1zaro\u2010Gaspar","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2312-6402","authenticated-orcid":false,"given":"Rosaura","family":"P\u00e9rez\u2010P\u00e9","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1306-8517","authenticated-orcid":false,"given":"Teresa","family":"Mui\u00f1o\u2010Blanco","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9842-6197","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Cebri\u00e1n\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1997-4262","authenticated-orcid":false,"given":"Adriana","family":"Casao","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular Facultad de Veterinaria Instituto de Investigaci\u00f3n de Ciencias Ambientales de Arag\u00f3n (IUCA) Universidad de Zaragoza Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731107001383"},{"key":"e_1_2_10_3_1","article-title":"Extrapineal melatonin: Sources, regulation, and potential functions","volume":"1","author":"Acu\u00f1a\u2010Castroviejo D.","year":"2014","journal-title":"Cellular and Molecular Life Sciences"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.1016\/S0021-9258(19)69420-2","article-title":"The reductive cleavage of myeloperoxidase in half, producing enzymically active hemi\u2010myeloperoxidase","volume":"256","author":"Andrews P. 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