{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T11:22:21Z","timestamp":1778066541375,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Mu"],"award-info":[{"award-number":["Mu"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["1520\/4\u20102"],"award-info":[{"award-number":["1520\/4\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Schi 476\/12\u20102"],"award-info":[{"award-number":["Schi 476\/12\u20102"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cryobanking of gametes in combination with artificial insemination is an essential option to support conservation programmes for endangered and threatened species. About two\u2010thirds of the felid species are classified as \u2018near threatened\u2019, \u2018vulnerable\u2019 or \u2018endangered\u2019 (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.cites.org\">www.cites.org<\/jats:ext-link>), and mostly, epididymal sperm are collected from euthanized or castrated male felids and cryopreserved. However, epididymal compared with ejaculated and cryopreserved compared with fresh sperm have a limited potential to fertilize if vaginal non\u2010surgical insemination is applied in feline species. Missing or highly diluted seminal fluid in epididymal and cryopreserved sperm, as well as a potential interference of extender ingredients with the natural interactive properties of sperm in the female genital tract is discussed as potential drawback which hampers a proper sperm transit and fertilization besides the limited longevity of cryopreserved feline sperm. Individual components in seminal fluid as well as cryoextenders may adversely alter sperm properties and have a different impact on fertility and preservation success. The identification and investigation of beneficial as well as detrimental components is a precondition to deduce options for improving the process of cryopreservation in felids, particularly, if only epididymal sperm are available.<\/jats:p>","DOI":"10.1111\/rda.13581","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T10:09:52Z","timestamp":1577354992000},"page":"55-60","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Assisted reproduction for felid species conservation\u2014Sperm competences at risk"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1749-4863","authenticated-orcid":false,"given":"Karin","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Department Reproduction Biology Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Eder","sequence":"additional","affiliation":[{"name":"Department Reproduction Biology Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Jakop","sequence":"additional","affiliation":[{"name":"Department Reproduction Biology Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Schiller","sequence":"additional","affiliation":[{"name":"Medical Faculty Institute of Medical Physics and Biophysics University of Leipzig Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Biology Humboldt\u2010University Berlin Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Bashawat","sequence":"additional","affiliation":[{"name":"Department Reproduction Biology Leibniz Institute for Zoo and Wildlife Research Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,26]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Paper presented at the Proc. 11th International Conference on Behaviour, Physiology and Genetics of Wildlife","author":"Bashawat M.","year":"2017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.022996"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.07.022"},{"key":"e_1_2_9_5_1","volume-title":"Paper presented at the Proc. 8th International Conference on Behaviour, Physiology and Genetics of Wildlife","author":"Eder S.","year":"2011"},{"key":"e_1_2_9_6_1","volume-title":"Paper presented at the Proc. 8th ISCFR and 19th EVSSAR congress","author":"Eder S.","year":"2016"},{"key":"e_1_2_9_7_1","first-page":"173","volume-title":"Biology of male germ cells","author":"Ekhlasi\u2010Hundrieser M.","year":"2008"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.08.023"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1990.tb01583.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.01.031"},{"key":"e_1_2_9_11_1","volume-title":"Studies of canine and feline sperm viability under different storage procedures with special reference to chilling, freezing, and use of zona pellucida binding assays","author":"Hermansson U.","year":"2006"},{"key":"e_1_2_9_12_1","volume-title":"Paper presented at the Proc. 11th International Congress on Animal Reproduction and Artificial Insemination","author":"Johnston S. D.","year":"1988"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12666"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2003.12.001"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.10.024"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-145-37757"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(77)80146-0"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12453"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18350"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12863"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01436.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0175"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.10.008"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2940001"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.06.011"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.13581","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13581","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13581","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13581","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T08:10:38Z","timestamp":1693815038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13581"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,26]]},"references-count":24,"journal-issue":{"issue":"S2","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1111\/rda.13581"],"URL":"https:\/\/doi.org\/10.1111\/rda.13581","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,26]]},"assertion":[{"value":"2019-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}