{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:27:07Z","timestamp":1790728027365,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,2]],"date-time":"2020-01-02T00:00:00Z","timestamp":1577923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760753"],"award-info":[{"award-number":["31760753"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non\u2010oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up\u2010regulated and 21 were down\u2010regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac\u2010related pathways, such as \u2018dilated cardiomyopathy\u2019, \u2018hypertrophic cardiomyopathy\u2019, \u2018cardiac muscle contraction\u2019 and \u2018adrenergic signalling in cardiomyopathy\u2019. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus.<\/jats:p>","DOI":"10.1111\/rda.13607","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T10:36:37Z","timestamp":1576492597000},"page":"189-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7964-9309","authenticated-orcid":false,"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze","family":"Gan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijiang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"},{"name":"College of Life Science and Technology Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5470-3815","authenticated-orcid":false,"given":"Xingxu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agriculture University Lanzhou China"},{"name":"College of Life Science and Technology Gansu Agriculture University Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,2]]},"reference":[{"issue":"4","key":"e_1_2_10_2_1","first-page":"257","article-title":"Cardiovascular abnormalities in Folr1 knockout mice and folate rescue \u2020","volume":"79","author":"\u2021 H. 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