{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:47:55Z","timestamp":1787626075030,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was designed to investigate the hormonal and metabolic factors associated with placental retention in buffaloes with respect to the roles of oxidative stress biomarkers and serotonin receptors. Blood samples were collected at weeks 3, 2 and 1 pre\u2010partum and at calving from 37 buffaloes; thirty normally dropped their placentae (Non\u2010RFM group) and 7 dropped their placentae after 12\u00a0hr post\u2010calving (RFM group). Serum progesterone (P<jats:sub>4<\/jats:sub>), oestradiol, cortisol, non\u2010esterified fatty acids (NEFA), beta\u2010hydroxybutyric acid (BHBA), antioxidant\/oxidant biomarkers and mineral concentrations were assessed. Histopathology and histochemistry were implemented to detect collagen in foetal placental tissues. Immunohistochemistry for serotonin receptors in placental tissues was performed. Significant elevations in P<jats:sub>4<\/jats:sub>, cortisol, NEFA, BHBA and oxidative biomarkers concentrations were observed in the RFM group. However, oestradiol, antioxidants and mineral concentrations were significantly lower in RFM buffaloes than Non\u2010RFM group. Histopathological examination revealed degenerative changes and necrosis in retained placental tissue compared with that in normal placental tissues. Serotonin receptors were significantly expressed with collagen condensation in retained placental tissues. Furthermore, inferior reproductive performance was pronounced in the retained group. In conclusion, retained foetal membranes in buffaloes were associated with hormonal imbalance, metabolic perturbation, oxidative stress, serotonin receptor upregulation and markedly reduced fertility indices.<\/jats:p>","DOI":"10.1111\/rda.13640","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T11:50:19Z","timestamp":1579607419000},"page":"469-478","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hormonal and metabolic profiles related to placental retention with emphasis on oxidative stress and serotonin receptors in pluriparous buffaloes"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3590-5953","authenticated-orcid":false,"given":"Doaa H.","family":"Elsayed","sequence":"first","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Suez Canal University Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8906-7970","authenticated-orcid":false,"given":"Heba M. A.","family":"Abdelrazek","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Veterinary Medicine Suez Canal University Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2060-9967","authenticated-orcid":false,"given":"Fakhri E.","family":"El\u2010Azzazi","sequence":"additional","affiliation":[{"name":"Department of Animal Production Faculty of Agriculture Suez Canal University Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimaa A. 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