{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:57:12Z","timestamp":1791442632758,"version":"4.3.3"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:00:00Z","timestamp":1589241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study examines the effect of L\u2010carnitine (LC) on chilled ram semen stored for up to 96\u00a0hr. Semen samples were collected, placed in a skimmed milk\u00a0+\u00a06% egg yolk extender, pooled, aliquoted and diluted with the same extender supplemented with different LC concentration: 0 (control), 1\u00a0mM (LC1), 2.5\u00a0mM (LC2.5), 5\u00a0mM (LC5), 7.5\u00a0mM (LC7.5) or10\u00a0mM (LC10). Sperm kinetics and membranes (plasma, acrosome and mitochondrial) were examined using the CASA system and triple fluorescence staining (PI\/ PNA\u2010FITC\/Mitotracker). The progressive motility was greater (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05) with LC7.5 treatment than the control sperm at 96\u00a0hr. The curvilinear velocity (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.01) and the percentage of sperm with intact membranes (plasma\/acrosome\/mitochondria) (\n                    <jats:italic>p\u00a0&lt;\u00a0<\/jats:italic>\n                    .01) were greater with all LC treatments than the control group at all times. Straight line velocity was greater (\n                    <jats:italic>p\u00a0&lt;\u00a0<\/jats:italic>\n                    .01) with LC5 and LC7.5 treatments than the control group after 48\u00a0hr. The LC5 group also returned lower ALH values (\n                    <jats:italic>p\u00a0&lt;\u00a0<\/jats:italic>\n                    .05) than these seen for the control groups after 48\u00a0hr. The fertilizing capacity of LC5 samples stored at 15\u00b0C for 2\u00a0hr (LC5\u201015\u00b0C\u20102h) and at 5\u00b0C for 24\u00a0hr (LC5\u20105\u00b0C\u201024h) was tested in three ewe groups via cervical fixed\u2010time artificial insemination. In two groups, the fertilizing capacity of the LC5\u20105\u00b0C\u201024h was reduced (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.001). In the remaining group, however, no significant difference was seen between the LC5\u201015\u00b0C\u20102h and LC5\u20105\u00b0C\u201024h sperm in this respect (pregnancy rates 52.4% versus 42.8%;\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a0.05). Overall, the present results suggest that supplementing skimmed milk\u2013egg yolk\u2010based extenders with LC has a positive effect on chilled sperm variables and fertilizing capacity.\n                  <\/jats:p>","DOI":"10.1111\/rda.13687","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T14:03:04Z","timestamp":1587391384000},"page":"805-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Supplementing a skimmed milk\u2013egg yolk\u2010based extender with L\u2010carnitine helps maintain the motility, membrane integrity and fertilizing capacity of chilled ram sperm"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0266-5431","authenticated-orcid":false,"given":"Diego A.","family":"Galarza","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction INIA Madrid Spain"},{"name":"Faculty of Agriculture Sciences University of Cuenca Cuenca Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"L\u00f3pez\u2010Sebasti\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction INIA Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Santiago\u2010Moreno","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction INIA Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60920-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0810459"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0730441"},{"issue":"2","key":"e_1_2_10_6_1","first-page":"77","article-title":"Effects of L\u2010carnitine and L\u2010acetyl\u2010carnitine on testicular sperm motility and chromatin quality","volume":"10","author":"Aliabadi E.","year":"2012","journal-title":"Iranian Journal of Reproductive 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