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Thirty\u2010six pluriparous ewes received progestogen sponge (60\u00a0mg) for 9\u00a0days plus eCG administration (300\u00a0IU i.m.) 24\u00a0hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16\u00a0hr before NSER 37.5\u00a0\u00b5g d\u2010cloprostenol i.m. and different doses of oestradiol benzoate: 0.0\u00a0mg (0EB group; <jats:italic>n<\/jats:italic>\u00a0=\u00a012); 0.5\u00a0mg (0.5EB group; <jats:italic>n<\/jats:italic>\u00a0=\u00a012) or 1.0\u00a0mg of oestradiol (1.0EB group, <jats:italic>n<\/jats:italic>\u00a0=\u00a012). All ewes received oxytocin (50\u00a0IU) i.v. 20\u00a0min before NSER, which was performed 8\u00a0days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24\u00a0hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post\u2010NSER fertility. NSER was performed in 91.7% (33\/36) of the animals with overall fluid recovery efficiency over 97% (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The cervical transposing with Hegar dilator was longer (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in 0EB (4.2\u00a0\u00b1\u00a00.3\u00a0min) compared to 0.5EB (1.7\u00a0\u00b1\u00a00.3\u00a0min) and 1.0EB group (1.5\u00a0\u00b1\u00a00.3\u00a0min). The cervical transposing with mandrel\/catheter was longer (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in 0EB (2.4\u00a0\u00b1\u00a00.5\u00a0min) than 1.0EB group (1.3\u00a0\u00b1\u00a00.5\u00a0min). Overall duration of uterine flushing was 25.4\u00a0min with structure recovery rate of 43.5%, with no difference among groups (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The post\u2010NSER fertility was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate.<\/jats:p>","DOI":"10.1111\/rda.13692","type":"journal-article","created":{"date-parts":[[2020,4,26]],"date-time":"2020-04-26T12:03:23Z","timestamp":1587902603000},"page":"844-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Successful transcervical uterine flushing can be performed without or reduced dose of oestradiol benzoate in cervical relaxation protocol in Dorper ewes"],"prefix":"10.1111","volume":"55","author":[{"given":"Jenniffer Hauschildt","family":"Dias","sequence":"first","affiliation":[{"name":"Universidade Federal de Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Am\u00e9lia","family":"Pupin","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Saloni","family":"Duarte","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Lopes","family":"Brair","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleber Jonas Carvalho","family":"de Paula","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio Paula","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Universidade Federal do Par\u00e1 Castanhal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ribrio Ivan Tavares Pereira","family":"Batista","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4872-1718","authenticated-orcid":false,"given":"Joanna Maria Gon\u00e7alves","family":"Souza\u2010Fabjan","sequence":"additional","affiliation":[{"name":"Universidade Federal Fluminense Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7730-290X","authenticated-orcid":false,"given":"Maria Em\u00edlia Franco","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Agr\u00e1rias e Veterin\u00e1rias Universidade Estadual Paulista Jaboticabal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8028-3984","authenticated-orcid":false,"given":"Jeferson Ferreira","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Embrapa Caprinos e Ovinos Coronel Pacheco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874255601103010162"},{"key":"e_1_2_10_3_1","volume-title":"Manual para exame androl\u00f3gico e avaliac\u00e3o de s\u00eamen animal","author":"Col\u00e9gio Brasileiro de Reprodu\u00e7\u00e3o Animal \u2013 CBRA","year":"2013"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13318"},{"key":"e_1_2_10_5_1","volume-title":"Hormones in Meat","author":"European Commission","year":"2017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.11.004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-41626783"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18324"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.04.025"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2012.08.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2019.02.004"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352009000200005"},{"issue":"1","key":"e_1_2_10_13_1","article-title":"Colheita Transcervical de Embri\u00f5es Ovinos da Ra\u00e7a Santa In\u00eas no Semi\u2010\u00e1rido Nordestino. 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