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The present research was conducted to study semen quality, some stress and inflammatory markers in <jats:italic>Ossimi<\/jats:italic> rams under both hot and mild climatic conditions. The current study was conducted on selected 46 ram samples divided into two groups during summer and winter. Semen analysis, testosterone (TES), cortisol (COR) and blood glucose (BG) levels, and lipid and protein profiles were done. Concentrations of tumour necrosis factor alpha (TNF\u2010\u03b1), tissue inhibitor of metalloproteinase\u20103 (TIMP\u20103), nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH) and specific activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were assessed. The results in summer compared to winter revealed significant elevation of total defects and number of dead sperms; however, there was reduction in sperm total motility and concentration and external epididymal tail duct diameter. Histological study of epididymal tail lumen exhibited azoospermia. Further, TES, TIMP\u20103 and GSH levels were decreased and COR, TNF\u2010\u03b1, NO and MDA were raised. Specific activities of GPx and SOD were also declined. Additionally, there was a significant increase in concentrations of BG and lipid profiles except high\u2010density lipoprotein. Our data concluded that there were new insights into TNF\u2010\u03b1 and TIMP\u20103 as biomarkers can be used in diagnosis of sheep suffering from HS, but further studies are recommended to do in future work about such aspect.<\/jats:p>","DOI":"10.1111\/rda.13697","type":"journal-article","created":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T14:05:40Z","timestamp":1589033140000},"page":"870-881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Impact of thermal stress exposure on seminal quality, antioxidant defence system, TNF\u2010\u03b1 and TIMP\u20103 in <i>Ossimi<\/i> ram"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2884-8148","authenticated-orcid":false,"given":"Marwa","family":"El\u2010Zeftawy","sequence":"first","affiliation":[{"name":"Biochemistry Department Faculty of Veterinary Medicine New Valley University New Valley Egypt"},{"name":"Biochemistry Department Faculty of Science Biological Screening and Preclinical Trial Lab Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gamal B.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Animal Production Department Faculty of Agriculture Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervat","family":"Hassan","sequence":"additional","affiliation":[{"name":"Theriogenology Department Faculty of Veterinary Medicine New Valley University New Valley Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2014.02.013"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-45"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0097-5"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-009-9210-9"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/aoas-2016-0068"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2019.09.003"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.02.034"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03420.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011-4675"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.12379"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.14737\/journal.aavs\/2014\/2.11.632.637"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00342"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-7714"},{"issue":"6","key":"e_1_2_11_15_1","first-page":"10","article-title":"Estimation of nitric oxide as an inflammatory marker in periodontitis","volume":"8","author":"Chavda M.","year":"2019","journal-title":"International Journal of Scientific Research"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23208"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01252.x"},{"issue":"3","key":"e_1_2_11_18_1","first-page":"558","article-title":"Purification and properties of rat lung soluble glutathione peroxidase. 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