{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T04:08:25Z","timestamp":1768795705140,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/18384\u20100"],"award-info":[{"award-number":["2017\/18384\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability. Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood. This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status. To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000\u00a0\u00b5g\/ml) and analysed for viscosity and adhesion strength characteristics. Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility). The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000\u00a0\u00b5g\/ml group in which the effect was more pronounced. No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities. However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa. In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell.<\/jats:p>","DOI":"10.1111\/rda.13739","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T13:35:32Z","timestamp":1591536932000},"page":"974-983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Changes in fertilization medium viscosity using hyaluronic acid impact bull sperm motility and acrosome status"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2646-9168","authenticated-orcid":false,"given":"Aldcejam Martins","family":"da Fonseca Junior","sequence":"first","affiliation":[{"name":"Center for Natural and Human Sciences Federal University of ABC Santo Andr\u00e9 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Gaita","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Produzioni Animali Universit\u00e0 degli Studi di Napoli Federico II Napoli Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Ricardo","family":"Argumedo","sequence":"additional","affiliation":[{"name":"Center for Natural and Human Sciences Federal University of ABC Santo Andr\u00e9 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia Signori","family":"de Castro","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7947-4027","authenticated-orcid":false,"given":"Jo\u00e3o Diego de Agostini","family":"Losano","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-8604","authenticated-orcid":false,"given":"Roberta","family":"Ferreira Leite","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0825","authenticated-orcid":false,"given":"Marcilio","family":"Nichi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5967-9316","authenticated-orcid":false,"given":"Mayra Elena Ortiz d\u2019\u00c1vila","family":"Assump\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9289-4229","authenticated-orcid":false,"given":"Daniele Ribeiro","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Center for Natural and Human Sciences Federal University of ABC Santo Andr\u00e9 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2615-8573","authenticated-orcid":false,"given":"Antonio Alvaro Ranha","family":"Neves","sequence":"additional","affiliation":[{"name":"Center for Natural and Human Sciences Federal University of ABC Santo Andr\u00e9 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-3066","authenticated-orcid":false,"given":"Marcella Pecora","family":"Milazzotto","sequence":"additional","affiliation":[{"name":"Center for Natural and Human Sciences Federal University of ABC Santo Andr\u00e9 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjas94-007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.12.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(86)90010-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8213071"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12410"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.02.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-16-0240"},{"key":"e_1_2_9_9_1","first-page":"81","article-title":"Theory of swimming filaments in viscoelastic media","volume":"25","author":"Fu H. 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