{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:27:51Z","timestamp":1778002071622,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001845","name":"Science for Equity, Empowerment and Development Division","doi-asserted-by":"publisher","award":["SR\/WOS\u2010A\/LS\u2010651\/2012"],"award-info":[{"award-number":["SR\/WOS\u2010A\/LS\u2010651\/2012"]}],"id":[{"id":"10.13039\/501100001845","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Estrogen and its receptors are essential for sexual development and reproduction. Oestrogen receptor alpha (ER\u03b1) is a nuclear receptor activated by the hormone oestrogen. In male, ER\u03b1 is encoded by the gene ESR1 (oestrogen receptor1) responsible for better fertility. ESR1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of ESR1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. The present study was undertaken to investigate the expression and presence of ESR1 gene in fertile and low\u2010fertile male goat breeds. We identified ESR1 gene through various molecular tools. Genotyping was carried out by high resonance melting analysis using Roche Light Cycler 480(LC\u2010480) system and found three different genotypes. Genotypic frequency\u2010AA (blue\u20100.67), BB(Red\u20100.2), AB(Green\u20100.08) with allele frequency A(0.71 and B (0.29). The predominance of this gene in head of epididymis in fertile bucks was confirmed by SDS\u2010PAGE, Western blotting and immunohistochemistry. From the results, we corroborated that the present study provides a useful and effective way to predict male fertility in goat breeds, which in turn increases the percentage of fertility in flock leading to more number of offspring in a kidding season.<\/jats:p>","DOI":"10.1111\/rda.13746","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T22:19:26Z","timestamp":1592000366000},"page":"1080-1092","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular expression and identification of caprine estrogen receptor gene 1 for fertility status in bucks"],"prefix":"10.1111","volume":"55","author":[{"given":"S","family":"Saraswat","sequence":"first","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SD","family":"Kharche","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PK","family":"Rout","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Pawaiya","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Gangwar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DK","family":"Swain","sequence":"additional","affiliation":[{"name":"Department of Physiology DUVASU Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Kaushik","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Goats Mathura Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2014.02.016"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00107-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0235"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(98)70335-7"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1530485"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.026328"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.1.90"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei439"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.08.008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2005.03.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1002\/j.1939-4640.1997.tb02437.x","article-title":"Estrogen receptor (alpha and beta) expression in the excurrent ducts of the adult male rat reproductive tract","volume":"18","author":"Hess R.","year":"1997","journal-title":"Journal of Andrology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020068224909"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.080366"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-019-04984-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.109.007542"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660802433131"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_19_1","unstructured":"Michael J. C. Hwang Stuart Soo I. Kalush F. &Winn\u2010Deen Emily S.(2008).Estrogen receptor alpha variants and methods of detection thereof. Patents scope WO2001062969 A2."},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0260165"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.4.1161"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.12.013"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1650359"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod67.4.1043"},{"key":"e_1_2_10_25_1","unstructured":"Rout P. K.(2004).Sample collection DNA isolation and agarose gel electrophoresis. Frontier molecular tools for animal genome research.CIRG Publication 1 7."},{"issue":"2","key":"e_1_2_10_26_1","first-page":"000158","article-title":"RNA in semen and sperm: Importance and transcriptome analysis in buck","volume":"3","author":"Saraswat S.","year":"2018","journal-title":"Veterinary Science Research"},{"key":"e_1_2_10_27_1","first-page":"49","article-title":"The effect of antioxidants supplementation to cryopreservation protocol on seminal attributes and sperm membrane characteristics in sirohi goat","volume":"2","author":"Saraswat S.","year":"2012","journal-title":"Journal of Physiology and Pharmacology Advances"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12774"},{"key":"e_1_2_10_29_1","first-page":"207","article-title":"Estrogen receptor 1 gene expression in caprine and its effect on fertility","volume":"17","author":"Saraswat S.","year":"2016","journal-title":"Iranian Journal of Veterinary Research"},{"key":"e_1_2_10_30_1","first-page":"3","article-title":"Effect of ICI 182780 on estrogen receptor expression, fluid absorption and sperm motility in the epididymis of the bonnet monkey","volume":"3","author":"Shayu D.","year":"2004","journal-title":"Reproductive Biology and Endocrinology"},{"key":"e_1_2_10_31_1","volume-title":"Statistical methods","author":"Snedecor G. 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