{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T16:02:35Z","timestamp":1773331355565,"version":"3.50.1"},"reference-count":74,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fertilization capacity of sex\u2010sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex\u2010sorted bull sperm. In this study, the washing medium and fertilization medium of sex\u2010sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20134<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20135<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20136<\/jats:sup>\u00a0M) or Lyc (0, 1\u00a0\u00d7\u00a010<jats:sup>\u20134<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20135<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20136<\/jats:sup>, 1\u00a0\u00d7\u00a010<jats:sup>\u20137<\/jats:sup>). After washing twice and incubation for 1.5\u00a0hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (\u0394\u03c8m) and IVF (in vitro fertilization) ability of sex\u2010sorted sperm were investigated. For the sex\u2010sorted sperm of bulls A, B and C, 1\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup>\u00a0M VC or 1\u00a0\u00d7\u00a010<jats:sup>\u20134<\/jats:sup>\u00a0M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their \u0394\u03c8m levels and cleavage rates after IVF. When blastocysts were concerned, 1\u00a0\u00d7\u00a010<jats:sup>\u20134<\/jats:sup>\u00a0M Lyc significantly improved the blastocyst rates and their IFN\u2010tau expression of bulls A and C. In conclusion, supplementation of 1\u00a0\u00d7\u00a010<jats:sup>\u20133<\/jats:sup>\u00a0M VC or 1\u00a0\u00d7\u00a010<jats:sup>\u20134<\/jats:sup>\u00a0M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex\u2010sorted bull sperm during IVF procedure.<\/jats:p>","DOI":"10.1111\/rda.13748","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T10:19:31Z","timestamp":1592302771000},"page":"1103-1114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex\u2010sorted bull sperm by inhibiting the oxidative stress"],"prefix":"10.1111","volume":"55","author":[{"given":"Pei\u2010Pei","family":"Zhang","sequence":"first","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong\u2010Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010sheng","family":"Hao","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u2010Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Hua","family":"Du","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-179X","authenticated-orcid":false,"given":"Yun\u2010Wei","family":"Pang","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan\u2010Jiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Ying","family":"Zou","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Hao","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sha","family":"Yang","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Min","family":"Ruan","sequence":"additional","affiliation":[{"name":"International Joint Center for Biomedical Innovation School of Life Sciences Henan University Kaifeng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010Ming","family":"Huang","sequence":"additional","affiliation":[{"name":"Dairy Cattle Research Center Shandong Academy of Agricultural Sciences Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng\u2010cheng","family":"Jiao","sequence":"additional","affiliation":[{"name":"Center of Biomedical Analysis Tsinghua University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua\u2010Bin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6110-2690","authenticated-orcid":false,"given":"Xue\u2010Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction &amp; Breeding Institute of Animal Science (IAS) Chinese Academy of Agricultural Sciences (CAAS) Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.1.183"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.68"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327119864160"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.12.027"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-008-9256-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)84066-6"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840110122"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.508"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(93)90219-U"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod12.2.260"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2009.10.012"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12301"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-017-1311-y"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1052"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.08.003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009253330451"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.126384"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.01.014"},{"key":"e_1_2_9_21_1","first-page":"103","article-title":"Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples","volume":"12","author":"Fanaei H.","year":"2014","journal-title":"Iranian Journal of Reproductive Medicine"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-9-100"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod57.6.1401"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.032"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00308-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1030017"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.15414\/jmbfs.2017\/18.7.3.242-247"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.04.180"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.160"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.5.1240"},{"issue":"12","key":"e_1_2_9_31_1","first-page":"791","article-title":"The protective effect of vitamin C on phenylhydrazine\u2010induced hemolytic anemia on sperm quality and in\u2010vitro embryo development in mice","volume":"16","author":"Karimipour M.","year":"2019","journal-title":"International Journal of Reproductive BioMedicine"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.10.006"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.2478\/intox-2013-0015"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8040096"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1097-5"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20163921"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25687-z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.10.2171"},{"key":"e_1_2_9_39_1","first-page":"357","article-title":"Oxidative stress & male infertility","volume":"129","author":"Makker K.","year":"2009","journal-title":"Indian Journal of Medical Research"},{"issue":"1","key":"e_1_2_9_40_1","first-page":"25","article-title":"Effect of lycopene on fowl sperm characteristics during in vitro storage","volume":"71","author":"Mangiagalli M. G.","year":"2007","journal-title":"Archiv fur Geflugelkunde"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2018.02.006"},{"issue":"10","key":"e_1_2_9_42_1","first-page":"598","article-title":"The effect of tomato\u2010derived lycopene on low carotenoids and enhanced systemic inflammation and oxidation in severe obesity","volume":"11","author":"Markovits N.","year":"2009","journal-title":"Israel Medical Association Journal"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.107.003459"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.04.005"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrefrig.2005.07.008"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb23761.x"},{"key":"e_1_2_9_47_1","unstructured":"J. K. O\u2019Brien E. G. Crichton K. M. Evans J. L. Schenk T. Stojanov G. Evans N. M. Loskutoff 2002"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.01.002"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01278.x"},{"key":"e_1_2_9_50_1","first-page":"30066","article-title":"Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5\u00b0C","volume":"16","author":"Peker akalin P.","year":"2016","journal-title":"Small Ruminant Research"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2004.00560.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.12654"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.09.027"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2018.08.006"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2012.03.006"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.2527\/1994.722434x"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327118816137"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2012-02304"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(94)01327-I"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12944"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00215-0"},{"key":"e_1_2_9_62_1","first-page":"214","volume-title":"Current conceptions","author":"Senger P. L.","year":"2005"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.12709"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0113-9"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.2754\/avb200776030383"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/19390211.2016.1211783"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2004.05.003"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1998.tb01178.x"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10907-9"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO02300J"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.01009.x"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.3923\/javaa.2010.3117.3124"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD19234"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145383"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2009.04.004"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.13748","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13748","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13748","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:07:42Z","timestamp":1693958862000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,6]]},"references-count":74,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/rda.13748"],"URL":"https:\/\/doi.org\/10.1111\/rda.13748","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,6]]},"assertion":[{"value":"2020-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}