{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T01:20:49Z","timestamp":1794446449603,"version":"4.9.1"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T00:00:00Z","timestamp":1596326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004360","name":"Sveriges Lantbruksuniversitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004360","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000593","name":"Society for Reproduction and Fertility","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Centrifugation of boar semen through one layer of 40% colloid (Porcicoll) was previously shown to separate spermatozoa from bacteria without having a detrimental effect on sperm quality. However, some spermatozoa were lost. The purpose of the present study was to determine whether 20% or 30% Porcicoll could be used to recover most of the spermatozoa without impacting on sperm quality. Insemination doses (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) from a commercial boar station were sent to the laboratory at the Swedish University of Agricultural Sciences and processed by Single Layer Centrifugation with 20% and 30% Porcicoll approximately 7\u00a0hr after semen collection. The resulting sperm samples and controls were evaluated for sperm quality immediately and again after storage at 16\u201318\u00b0C for 4 and 7\u00a0days. Sperm recovery was 94\u00a0\u00b1\u00a018% and 87\u00a0\u00b1\u00a015% for 20% and 30% Porcicoll, respectively (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). Sperm mitochondrial membrane potential and chromatin integrity were unaffected (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The proportion of live spermatozoa producing superoxide (9\u00a0\u00b1\u00a08%, 7\u00a0\u00b1\u00a06% and 3\u00a0\u00b1\u00a01%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), and the proportion of spermatozoa with high stainability DNA (0.68\u00a0\u00b1\u00a019%, 0.61\u00a0\u00b1\u00a00.22% and 0.96\u00a0\u00b1\u00a00.23%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05\u2010 &lt;0.01), were marginally increased whereas membrane integrity, although high, was lower in the centrifuged samples than in the controls (82\u00a0\u00b1\u00a08%, 83\u00a0\u00b1\u00a05% versus 92\u00a0\u00b1\u00a04%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, centrifugation through 20% or 30% Porcicoll enables most spermatozoa to be recovered, without having a major effect on sperm quality. These results are encouraging for further studies involving microbiological investigation of the processed samples, and scaling\u2010up to process larger volumes of boar ejaculates.<\/jats:p>","DOI":"10.1111\/rda.13779","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T20:48:45Z","timestamp":1595278125000},"page":"1337-1342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Single Layer Centrifugation with 20% or 30% Porcicoll separates the majority of spermatozoa from a sample without adversely affecting sperm quality"],"prefix":"10.1111","volume":"55","author":[{"given":"Sourabh","family":"Deori","sequence":"first","affiliation":[{"name":"Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"},{"name":"ICAR Research Complex for North Eastern Hill Region Umiam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4777-5490","authenticated-orcid":false,"given":"Anders","family":"Johannisson","sequence":"additional","affiliation":[{"name":"Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5245-7331","authenticated-orcid":false,"given":"Jane","family":"Morrell","sequence":"additional","affiliation":[{"name":"Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,2]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"IMV\u2010Technologies. 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