{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:57:16Z","timestamp":1747187836466,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro\u2010produced (IVP) embryos are reported to be developmentally lesser competent than in vivo\u2010derived (IVD) embryos and supposed to differ in the expression of genes related with glucose metabolism. So, the present study was conducted to analyse the expression pattern of <jats:italic>GLUT 1, 5, 8<\/jats:italic> and <jats:italic>citrate synthase<\/jats:italic> (<jats:italic>CS<\/jats:italic>) in oocytes and embryos produced in vivo or in vitro in buffalo. IVD embryos were obtained from 18 superovulated buffaloes. IVP embryos were obtained from slaughterhouse\u2010derived oocytes subsequently subjected to in vitro fertilization and culture. Total RNA was isolated from different stages of oocytes (immature and in vitro matured) and embryos (8\u201316 cell to blastocysts of IVP embryos and morula to blastocysts of IVD embryos). Results demonstrated that the expression of <jats:italic>GLUT1, GLUT 8<\/jats:italic> increased from 8 to 16 cells to blastocyst and was significantly (<jats:italic>p<\/jats:italic>\u00a0&lt; .05) higher in IVP embryos. Expression of both genes was (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) higher in IVD than in IVP blastocysts; though <jats:italic>GLUT5<\/jats:italic> transcripts were not detected at 8\u2010 to 16\u2010cell stage IVP embryos, significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) higher transcripts were found at morula and blastocyst stages irrespective of embryo source with significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) higher expression in IVD embryos compared to IVP embryos. No significant difference was observed in <jats:italic>citrate synthase<\/jats:italic> expression in embryos at morula stage irrespective of the embryo source while significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) higher transcript level was observed at blastocyst stage with no difference between in vivo and in vitro embryos. It can be concluded that expression of <jats:italic>GLUTs<\/jats:italic> and <jats:italic>CS<\/jats:italic> is upregulated with progression of embryonic stage and expression is higher in in vivo embryos than in vitro counter parts; thus, it can be said that in vivo<jats:italic>\u2010<\/jats:italic>produced embryos are metabolically superior to in vitro embryos.<\/jats:p>","DOI":"10.1111\/rda.13782","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T14:21:25Z","timestamp":1595600485000},"page":"1362-1370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression pattern of GLUT 1, 5, 8 and citrate synthase transcripts in buffalo (<i>Bubalus bubalis<\/i>) preimplantation embryos produced in vitro and derived in vivo"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5052-9477","authenticated-orcid":false,"given":"Gutulla","family":"Taru Sharma","sequence":"first","affiliation":[{"name":"Division of Physiology and Climatology ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8833-7414","authenticated-orcid":false,"given":"Amar","family":"Nath","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiv","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics GBPUA &amp;T Pantnagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumit","family":"Singhal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Gynaecology and Obstetrics College of Veterinary Science GADVASU Ludhiana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2142-3024","authenticated-orcid":false,"given":"Vikash","family":"Chandra","sequence":"additional","affiliation":[{"name":"Division of Physiology and Climatology ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gutulla","family":"Saikumar","sequence":"additional","affiliation":[{"name":"Division of Pathology ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,17]]},"reference":[{"issue":"6","key":"e_1_2_10_2_1","first-page":"3412","article-title":"Multiple facilitated glucose transporters SLC2As are required for normal mouse preimplantation embryo development","volume":"11","author":"Arhin S. 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