{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:55:51Z","timestamp":1771300551325,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T00:00:00Z","timestamp":1601078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of the study was to evaluate the interval from onset of oestrus to time of artificial insemination (AI) to obtain the optimum pregnancy rate with sex\u2010sorted semen in Holstein heifers. Heifers in oestrus were detected and inseminated only by using heat\u2013rumination neck collar comprised electronic identification tag at the age of 13\u201314\u00a0months. Heifers (<jats:italic>n<\/jats:italic>\u00a0=\u00a0283) were randomly assigned to one of three groups according to the timing of insemination at 12\u201316\u00a0hr (G1, <jats:italic>n<\/jats:italic>\u00a0=\u00a097), at 16.1\u201320\u00a0hr (G2, <jats:italic>n<\/jats:italic>\u00a0=\u00a094) and at 20.1\u201324\u00a0hr (G3, <jats:italic>n<\/jats:italic>\u00a0=\u00a092) after reaching the activity threshold. The mean duration of oestrus was 18.6\u00a0\u00b1\u00a00.1\u00a0hr, and mean peak activity was found at 7.5\u00a0\u00b1\u00a00.1\u00a0hr after activity threshold. The mean interval from activity threshold to ovulation was 29.4\u00a0\u00b1\u00a00.4\u00a0hr. The overall pregnancy per AI (P\/AI) was 53.0% at 29\u201335\u00a0days and 50.9% at 60\u201366\u00a0days after AI. There was a significant reduction between G1 (13.8\u00a0\u00b1\u00a01.4\u00a0hr) and G3 (7.9\u00a0\u00b1\u00a01.4\u00a0hr) related to the intervals from AI to ovulation time. Sex\u2010sorted semen resulted in significantly higher P\/AI at 29\u201335\u00a0days when heifers inseminated in G3 (60.9%) after oestrus than those inseminated in G1 (49.5%) and G2 (48.9%). In terms of fertility, when the temperature\u2013humidity index (THI) was below the threshold value (THI \u226465) at the time of AI, there was a tendency (\u226465; 57.2% vs.\u00a0&gt;\u00a065; 47.1%) for high pregnancy rate. There was no effect of sire on P\/AI. In addition, the interaction of the technician with the time of AI was found significant, and three\u2010way interaction of technician, sire and time of AI was tended to be significant on pregnancy rate. Thus, in addition to delaying the time of insemination (between 20.1 and 24\u00a0hr) after oestrous detection, THI and experienced technician were also found to be critical factors in increasing fertility with the use of sex\u2010sorted semen in Holstein heifers.<\/jats:p>","DOI":"10.1111\/rda.13789","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T10:13:32Z","timestamp":1596449612000},"page":"1411-1417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effect of delaying the time of insemination with sex\u2010sorted semen on pregnancy rate in Holstein heifers"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6414-6752","authenticated-orcid":false,"given":"Baris","family":"Guner","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Bal\u0131kesir Bal\u0131kesir 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