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Previous studies have demonstrated that peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3) deficiency causes defects in the uteroplacental vasculature and induces embryonic losses in mice. However, its role in porcine placental angiogenesis remains unclear. In the present study, PPAR\u03b3 expression was investigated in porcine uteroplacental tissues at gestational day (GD) 25, GD40 and GD70 via quantitative polymerase chain reaction (qPCR), Western blot and immunohistochemistry (IHC). Moreover, the roles of PPAR\u03b3 in porcine placental angiogenesis were investigated using a cell model of porcine umbilical vein endothelial cells (PUVECs) to conduct proliferation, migration and tube formation assays in vitro and a mouse xenograft model to assess capillary formation in vivo. The results showed that PPAR\u03b3 was mainly located in the glandular epithelium, trophoblast, amniotic chorion epithelium and vascular endothelium, as indicated by the higher expression levels at GD25 and GD40 than at GD70 in endometrium and by higher expression levels at GD40 and GD70 than at GD25 in placenta. Moreover, PPAR\u03b3 expression was significantly downregulated in placenta with dead foetus. In PUVECs, knocking out PPAR\u03b3 significantly inhibited proliferation, migration and tube formation in vitro and inhibited capillary formation in mouse xenografts in vivo by blocking S\u2010phase, promoting apoptosis and downregulating the angiogenic factors of VEGF and its receptors. Overall, the spatiotemporal heterogeneity of PPAR\u03b3 expression in porcine uteroplacental tissue suggests its vital role in endometrial remodelling and placental angiogenesis, and PPAR\u03b3 regulates placental angiogenesis through VEGF\u2010mediated signalling.<\/jats:p>","DOI":"10.1111\/rda.13797","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T01:47:58Z","timestamp":1596764878000},"page":"1479-1489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Spatiotemporal heterogeneity of PPAR\u03b3 expression in porcine uteroplacenta for regulating of placental angiogenesis through VEGF\u2010mediated signalling"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0213-0365","authenticated-orcid":false,"given":"Juzuo","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biological and Food Engineering \u2018Double First\u2010Class\u2019 Applied Characteristic Discipline of Bioengineering in Hunan High Educational Institution Huaihua University Huaihua China"},{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqun","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"},{"name":"Huaihua Municipal Center of Animal Husbandry and Aquatic Products Bureau Huaihua China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhilong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisha","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwen","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Clinic Veterinary Medicine College of Veterinary Medicine Hunan Agricultural University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfei","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Biological and Food Engineering \u2018Double First\u2010Class\u2019 Applied Characteristic Discipline of Bioengineering in Hunan High Educational Institution Huaihua University Huaihua China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3159"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80209-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2013.11.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/684159"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2018.12.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012515400-0\/50010-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705051"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12093"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0705.2002.00731_2.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/rp.57.1.221"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092380204"},{"key":"e_1_2_9_13_1","first-page":"e51312","article-title":"Endothelial cell tube formation assay for the in vitro study of angiogenesis","volume":"91","author":"DeCicco\u2010Skinner K. 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