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However, the potential reducing effects of PT on equatorial lipid accumulation and ROS have not yet been elucidated for in vitro\u2010derived bovine embryos. The present study evaluated the effects of concentrations of 3, 1, 0.33, 0.11\u00a0\u03bcM PT, and a vehicle group on the percentage of cleaved embryos, embryos with more than 6 cells, percentage of blastocyst on Day 7 and 8, percentage of transferable embryos on Day 7, the cell count and relative concentration of lipids. In the second experiment, the effects of 0.33\u00a0\u03bcM PT and a vehicle group within two different O<jats:sub>2<\/jats:sub> environments (5% and 20%) were evaluated for ROS generation and the percentage of Day 8 blastocysts. In the first experiment, no significant differences were found between the treatments with PT and the vehicle group (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) concerning the percentage of cleaved embryos and embryos with more than 6 cells. Lipid reduction was observed in the groups treated with PT versus the vehicle group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The vehicle group showed a higher rate of blastocyst production on Days 7 and 8 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and an increase in the percentage of transferable embryos on Day 7 compared to the PT treatment groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Cell counts were not significantly different between treatments with PT and the vehicle group (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). In the second experiment, the O<jats:sub>2<\/jats:sub> concentration did not significantly affect ROS generation (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05); however, the groups treated with PT (0.33\u00a0\u03bcM) had a reduction in ROS (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The O<jats:sub>2<\/jats:sub> concentration also did not significantly affect the rate of blastocyst production on Day 8 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.7696). Future research should be conducted to ascertain whether the reduction of lipids could enhance the cryopreservation and post\u2010thaw viability of PT\u2010treated embryos.<\/jats:p>","DOI":"10.1111\/rda.13798","type":"journal-article","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T01:04:12Z","timestamp":1596848652000},"page":"1490-1500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of pterostilbene on development, equatorial lipid accumulation and reactive oxygen species production of in vitro\u2010produced bovine embryos"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2768-1881","authenticated-orcid":false,"given":"Froylan","family":"Sosa","sequence":"first","affiliation":[{"name":"Facultad de Estudios Superiores Cuautitl\u00e1n UNAM Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7450-8470","authenticated-orcid":false,"given":"Salvador","family":"Romo","sequence":"additional","affiliation":[{"name":"Facultad de Estudios Superiores Cuautitl\u00e1n UNAM Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Kjelland","sequence":"additional","affiliation":[{"name":"Conservation, Genetics &amp; Biotech, LLC Valley City ND USA"},{"name":"Mayville State University Mayville ND USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7200-8912","authenticated-orcid":false,"given":"Horacio","family":"\u00c1lvarez\u2010Gallardo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos INIFAP Tepatitl\u00e1n Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7853-7372","authenticated-orcid":false,"given":"Sandra","family":"P\u00e9rez\u2010Reynozo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos INIFAP Tepatitl\u00e1n Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0820-8052","authenticated-orcid":false,"given":"David","family":"Urb\u00e1n\u2010Duarte","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos INIFAP Tepatitl\u00e1n Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1215-0191","authenticated-orcid":false,"given":"Jose Fernando","family":"De La Torre\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Recursos Gen\u00e9ticos INIFAP Tepatitl\u00e1n Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RDv25n1Ab132"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00670-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3923\/rji.2010.129.145"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-112"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)04948-8"},{"key":"e_1_2_9_7_1","first-page":"314","article-title":"The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization","volume":"45","author":"Attaran M.","year":"2000","journal-title":"International Journal of Fertility and Women's Medicine"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD10148"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod27.1.147"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf2000103"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60038-7"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD05064"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD05063"},{"key":"e_1_2_9_14_1","first-page":"25","article-title":"Serum in synthetic oviduct fluid causes mitochondrial degeneration in ovine embryos","volume":"13","author":"Dorland M. 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