{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:20:21Z","timestamp":1792466421328,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T00:00:00Z","timestamp":1599350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infectious diseases and aetiological agents related to female reproductive systems were extensively covered compared to its male counterpart. There needs a proper study to bridge this gap, where microflora and infectious agents of both male and female reproductive are mutually intelligible. With this study, we aimed to evaluate the microbial contamination of the preputial cavity and also screened for abortion\u2010causing agents which are zoonotic as well. In goats, such types of abortions are caused by <jats:italic>Brucella melitensis<\/jats:italic>, <jats:italic>Chlamydophila<\/jats:italic>, <jats:italic>Campylobacter<\/jats:italic> and <jats:italic>Coxiella etc<\/jats:italic>. One of the major sources of contamination of semen is the preputial cavity, which is exposed to the external environment leading to spread of infection into the female via semen straws or by natural service. In the current study, good quality bucks (<jats:italic>n<\/jats:italic>\u00a0=\u00a032, Barbari\u00a0=\u00a012, Jamunapari\u00a0=\u00a010, Jakhrana\u00a0=\u00a010) which were routinely used for semen collection were screened for their preputial swabs, for the presence of the above pathogens. For detection of <jats:italic>Brucella melitensis<\/jats:italic>, OMP31 based TaqMan\u00ae probe real\u2010time PCR assay was used, and for Chlamydia, 16srRNA gene based SYBR\u00ae green real\u2010time PCR assay was employed for detection of <jats:italic>Chlamydophila abortus<\/jats:italic>. While for <jats:italic>Campylobacter<\/jats:italic> spp. and <jats:italic>Coxiella burnetii<\/jats:italic>, 16srRNA gene based conventional PCR and Trans\u2010PCR were used, respectively. In the current study, of the screened preputial swabs, none of them showed positive for <jats:italic>Brucella<\/jats:italic> and <jats:italic>Coxiella<\/jats:italic>, but of the screened 32 samples 17 showed positive for Chlamydia (53.13%) and two (6.25%) showed positive for <jats:italic>Campylobacter<\/jats:italic> spp. The current study emphasizes on the farms and laboratories which were regularly involved in screening of brucellosis also often overlook the other potential non\u2010brucella pathogens, causing abortions eventually incurring severe economic losses to the goat keepers.<\/jats:p>","DOI":"10.1111\/rda.13801","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T05:02:44Z","timestamp":1597381364000},"page":"1520-1525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular detection of important abortion\u2010causing microorganisms in preputial swab of breeding bucks using PCR\u2010based assays"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0826-0811","authenticated-orcid":false,"given":"Chetna","family":"Gangwar","sequence":"first","affiliation":[{"name":"AP&amp;R Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gururaj","family":"Kumaresan","sequence":"additional","affiliation":[{"name":"Animal Health Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Mishra","sequence":"additional","affiliation":[{"name":"Animal Health Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshuman","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Genetics and Breeding Faculty of Veterinary and Animal Sciences (FVAS) RGSC Banaras Hindu University Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Pachoori","sequence":"additional","affiliation":[{"name":"Animal Health Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5813-4333","authenticated-orcid":false,"given":"Sonia","family":"Saraswat","sequence":"additional","affiliation":[{"name":"AP&amp;R Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendra Pratap","family":"Singh","sequence":"additional","affiliation":[{"name":"Animal Health Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4392-2569","authenticated-orcid":false,"given":"Suresh Dinkar","family":"Kharche","sequence":"additional","affiliation":[{"name":"AP&amp;R Division ICAR\u2010 Central Institute for Research on Goats, Makhdoom, Farah Mathura India"},{"name":"Animal Genetics and Breeding Faculty of Veterinary and Animal Sciences (FVAS) RGSC Banaras Hindu University Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13126"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01650-18"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03253-8"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00234"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Waddliachondrophila, a Chlamydia\u2010related bacterium, has a negative impact on human spermatozoa","volume":"33","author":"Baud D.","year":"2017","journal-title":"Human Reproduction"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2017.964-972"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00178-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)00709-3"},{"issue":"1","key":"e_1_2_10_10_1","first-page":"48","article-title":"Bacterial and fungaltypes and their load in frozen semen of buffalo bulls","volume":"73","author":"Gangadhar K. 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