{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:50:19Z","timestamp":1784231419718,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T00:00:00Z","timestamp":1599350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31960659"],"award-info":[{"award-number":["31960659"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20190301037NY"],"award-info":[{"award-number":["20190301037NY"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>SUMOylation is a dynamic post\u2010translational modification process. However, the function of small ubiquitin\u2010like modifiers (SUMOs) in the maturation of porcine oocytes and embryo growth is not well known. Therefore, the aim of this study was to investigate the effect of E2 binding enzyme UBC9 on the expression of SUMO\u20101 protein during the in vitro maturation of porcine oocytes and embryo development after in vitro fertilization. Four groups were used: 0 (Control), 5, 10 and 15\u00a0\u00b5g\/ml UBC9. Western blotting, flow cytometry and RT\u2010qPCR were used to detect the in vitro maturation of porcine oocytes, SUMO\u20101 content, viability and the expression of apoptotic genes. Compared to those in the control treatment, the maturation rate (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and viability (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) of oocytes in the 5\u00a0\u03bcg\/ml treatment group decreased significantly. SUMO\u20101 protein markers appeared at 59 and 71\u00a0kDa and the content of SUMO\u20101 protein in the 10\u00a0\u00b5g\/ml treatment group decreased significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In the expression of apoptosis\u2010related genes, Bcl\u20102 gene expression was significantly downregulated in the 10\u00a0\u03bcg\/ml treatment group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). However, Bax and Caspase\u20103 were significantly upregulated in the 5\u00a0\u03bcg\/ml treatment group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). During embryonic development, the cleavage rate of oocytes in the 10\u00a0\u00b5g\/ml treatment group was significantly reduced (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), whereas blastocyst formation rate in the 5\u00a0\u00b5g\/ml treatment group was significantly reduced. UBC9 regulates SUMO\u20101 content in mature pig oocytes in vitro, which affects oocyte maturation rate, viability, apoptotic genes expression and embryo development after fertilization.<\/jats:p>","DOI":"10.1111\/rda.13802","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T08:35:32Z","timestamp":1597134932000},"page":"1526-1534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of E2 binding enzyme UBC9 on porcine oocyte maturation, apoptosis and embryo development"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6159-7109","authenticated-orcid":false,"given":"Da","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"},{"name":"Jilin city Academy of Agricultural Sciences Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuliang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Bi","sequence":"additional","affiliation":[{"name":"College of Life Sciences Xiamen University Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Life Sciences Xiamen University Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4948-9914","authenticated-orcid":false,"given":"Yanqiu","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1794-7017","authenticated-orcid":false,"given":"Yi","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1227104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00391"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.08.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.2.268"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2293"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2016.00103"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01849-7"},{"issue":"1","key":"e_1_2_10_9_1","first-page":"1690","article-title":"Nox1 activity inhibits reactive oxygen species generation and apoptosis in diabetic retinopathy","volume":"17","author":"Hu J. 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