{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:46:22Z","timestamp":1792597582662,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our previous study showed that intrauterine\u2010infused lipopolysaccharide (LPS) can be translocated to the mammary gland to induce weak inflammation. This study aimed to determine whether dexamethasone treatment facilitated the translocation of LPS from the uterus to the mammary gland to induce a heavy inflammatory response. Sixteen goats were divided into control and LPS groups, subjected to daily dexamethasone administration before saline or LPS infusion. Milk and blood samples were collected before and after LPS infusion to determine the milk yield and somatic cell count (SCC) and blood leucocyte count (BLC), cytokines, antimicrobial peptides and serum amyloid A (SAA) concentrations. Mammary gland tissues were collected from two goats before and 24\u00a0hr after LPS infusion for immunohistochemical analysis of LPS. The mean SCC in the LPS group was significantly higher, whereas the milk yield was significantly lower than that in the control group after LPS infusion. The mean BLC in the LPS group was significantly lower than in the control group after LPS infusion. Furthermore, milk concentrations of IL\u20101\u03b2, S100A8 and lactoferrin were higher in the LPS group than in the control group after infusion. LPS was detected in the connective tissues and inner alveolar spaces of the mammary glands 24\u00a0hr after LPS infusion. We concluded that dexamethasone administration facilitated the translocation of intrauterine\u2010infused LPS to the mammary gland, where it induced an inflammatory response. Therefore, LPS translocated from other organs, such as the uterus, can induce heavy inflammation in the mammary gland under immunosuppressive conditions.<\/jats:p>","DOI":"10.1111\/rda.13820","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T20:01:13Z","timestamp":1601323273000},"page":"1688-1697","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Translocation of intrauterine\u2010infused bacterial lipopolysaccharides to the mammary gland in dexamethasone\u2010treated goats"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9483-3876","authenticated-orcid":false,"given":"Fika Yuliza","family":"Purba","sequence":"first","affiliation":[{"name":"Graduate School of Biosphere Science Hiroshima University  Higashi\u2010Hiroshima Japan"},{"name":"Veterinary Medicine Study Program Faculty of Medicine Hasanuddin University  Makassar Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8496-690X","authenticated-orcid":false,"given":"Takahiro","family":"Nii","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8186-4481","authenticated-orcid":false,"given":"Yukinori","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9230-6346","authenticated-orcid":false,"given":"Naoki","family":"Isobe","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Higashi\u2010Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029906002305"},{"key":"e_1_2_10_3_1","first-page":"176","article-title":"Inhibition by glucocorticoids of the formation of interleukin\u20101 alpha, interleukin\u20101 beta, and interleukin\u20106: Mediation by decreased mRNA stability","volume":"43","author":"Amano Y.","year":"1993","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-009-9433-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(98)75591-2"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0940557"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.1999.049"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(02)00145-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2003030"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1186\/s12917-016-0907-1","article-title":"Lipopolysaccharide derived from the digestive tract triggers an inflammatory response in the uterus of mid\u2010lactating dairy cows during SARA","volume":"12","author":"Bilal M. 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