{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T19:11:33Z","timestamp":1787080293554,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was conducted on Hardhenu cattle to screen genomic region of leptin gene with an objective to find the association of genotypes with fertility and production traits. The association analysis with traits under study was analysed by least squares analysis of variance by taking SNPs genotype as fixed effects in the statistical model. The genotypic frequencies with respect to targeted loci g.92450765 G\u00a0&gt;\u00a0A indicated that AG (0.54) genotype was highest in Hardhenu cattle. Chi\u2010squared tests showed that g.92450765G\u00a0&gt;\u00a0A SNP meet with the Hardy\u2013Weinberg equilibrium (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05).The association analysis revealed significant association of genotypes with total milk yield (TMY) and 305\u00a0days milk yield (MY) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Service period (SP) and calving interval (CI) were also found significantly associated with genotypes (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Whereas, lactation length (LL), dry days (DD) and age at first calving (AFC) did not divulge any significant association with genotype. The AG and GG genotypes were associated with higher milk yields as compared to AA genotype, indicating that allele G was associated with superior milk performance. However, AA genotyped cattle found to be favourable with SP, CI and artificial insemination (AI) per conception compared to AG and GG genotyped cows. Chi\u2010square analysis revealed that genetic variants of g.92450765 G\u00a0&gt;\u00a0A SNP of leptin gene differ significantly with regard to reproductive disorders incidence (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The frequency of GG genotype (88.89%) in the affected animal group was very high followed by AG. The animals with GG genotype were found to be more susceptible to reproductive disorders as suggested by the higher odd ratio value (16.00) in logistic model. These observations and their differential association with the fertility and production traits can be utilized as an aid to selection for genetic improvement of antagonistic traits in dairy cows.<\/jats:p>","DOI":"10.1111\/rda.13826","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T14:01:36Z","timestamp":1600869696000},"page":"1698-1705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of candidate genotype of leptin gene associated with fertility and production traits in Hardhenu (<i>Bos taurus<\/i>\u00a0\u00d7\u00a0<i>Bos indicus<\/i>) cattle"],"prefix":"10.1111","volume":"55","author":[{"given":"Tejwanti","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-6421","authenticated-orcid":false,"given":"Ankit","family":"Magotra","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh C.","family":"Bangar","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha Rani","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Livestock Farm Complex LUVAS  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram","family":"Jeet","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baljit S.","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)  Hisar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"91","article-title":"Association of number of artificial inseminations per pregnancy in holstein dairy cows with polymorphism in luteinizing hormone receptor and follicle stimulating hormone receptor genes","volume":"54","author":"Arslan K.","year":"2017","journal-title":"Slovenian Veterinary Research"},{"key":"e_1_2_10_3_1","first-page":"1281","article-title":"Associations of PIT1 gene polymorphism with milk yield and composition traits in brown Swiss cattle","volume":"23","author":"Aytekin I.","year":"2013","journal-title":"Journal of Animal and Plant Science"},{"issue":"2","key":"e_1_2_10_4_1","first-page":"39","article-title":"Genetic polymorphism of leptin gene using PCR\u2010RFLP method in three different populations","volume":"45","author":"Azari M. 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