{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:04:40Z","timestamp":1756994680316,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","award":["NORTE\u201008\u20105369\u2010FSE\u2010000040"],"award-info":[{"award-number":["NORTE\u201008\u20105369\u2010FSE\u2010000040"]}],"id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>B\u00edsaro pig (BP), like many other local breeds reared in extensive non\u2010industrial systems, faces many constraints to comply with the EU welfare regulation, particularly regarding the restrictions to surgical castration. In order to adapt an immunocastration protocol to overtake this issue, a puberty timeline is needed. Using gonadal morphometry data from 91 young male BP, this study intended to characterize testicular development, describing the prominent cell types and structures, to ultimately assess the age at puberty in male BP through a mixed prediction model. As expected, the relations between several macro and microscopic parameters and their relation with age were as described within the literature. Post\u2010pubertal animals have larger and heavier gonads, lower Sertoli cell density\/tubule, higher Leydig cell density and larger seminiferous tubules. Meiosis was firstly seen in 44\u2010day\u2010old animals, elongated spermatids in 70\u2010day\u2010old animals. Complete spermatogenesis was firstly identified in a 90\u2010day\u2010old animal. Spermatozoa were present in the epididymis of 23 animals, aged from 70 to 240\u00a0days old, and in the vas deferens of 14 animals (105 to 240\u00a0days old). The prediction model inferred that male B\u00edsaro pigs reach puberty between 14 and 17\u00a0weeks (3 and 4\u00a0months old) and become sexually capable from 15 to 19\u00a0weeks (3.5 and 4.4\u00a0months old). These parameters confirm the sexual precocity of this breed.<\/jats:p>","DOI":"10.1111\/rda.13827","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T01:53:59Z","timestamp":1600912439000},"page":"1706-1713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of gonadal macroscopic and microscopic morphometry reveals precocious puberty in B\u00edsaro pig"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9753-8370","authenticated-orcid":false,"given":"Gustavo","family":"Paix\u00e3o","sequence":"first","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV) Universidade de Tr\u00e1s\u2010os\u2010Montes e Alto Douro  Vila Real Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6769-1844","authenticated-orcid":false,"given":"Alexandra","family":"Esteves","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV) Universidade de Tr\u00e1s\u2010os\u2010Montes e Alto Douro  Vila Real Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9079-7380","authenticated-orcid":false,"given":"Nuno","family":"Carolino","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria Unidade Estrat\u00e9gica de Investiga\u00e7\u00e3o e Servi\u00e7os de Biotecnologia e Recursos Gen\u00e9ticos  Vale de Santar\u00e9m Portugal"},{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA) Universidade de Lisboa  Lisboa Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9473-5283","authenticated-orcid":false,"given":"Maria","family":"dos Anjos Pires","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV) Universidade de Tr\u00e1s\u2010os\u2010Montes e Alto Douro  Vila Real Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5225-4510","authenticated-orcid":false,"given":"Rita","family":"Payan\u2010Carreira","sequence":"additional","affiliation":[{"name":"MED \u2010 Mediterranean Institute for Agriculture, Environment and Development Universidade de \u00c9vora  \u00c9vora Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod28.4.902"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.053835"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/090647099424169"},{"issue":"22","key":"e_1_2_10_5_1","first-page":"47","article-title":"Pubertad, grasa corporal y leptina en cerdas ib\u00e9ricas","author":"A\u00f1over P. G.","year":"2009","journal-title":"S\u00f3lo Cerdo Ib\u00e9rico"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.014"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.njas.2015.11.002"},{"volume-title":"Determining the Onset of Reproductive Capacity in Free\u2010Roaming, Unowned Cats","year":"2016","author":"Bohrer E.","key":"e_1_2_10_8_1"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40813-016-0046-x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20758"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1978.tb00603.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.08.010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1629"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.02.019"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2018.05.008"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"2","article-title":"Testicular biopsy score count\u2013a method for registration of spermatogenesis in human testes: Normal values and results in 335 hypogonadal males","volume":"1","author":"Johnsen S. G.","year":"1970","journal-title":"Hormones"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623316673495"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.02.009"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.10.009"},{"key":"e_1_2_10_20_1","first-page":"181","article-title":"Physiology of the Meishan boar","volume":"52","author":"Lunstra D. D.","year":"1997","journal-title":"Journal of Reproduction and Fertility"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2014.09.013"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547582"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.1996.tb00442.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863-016-0352-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","unstructured":"Paix\u00e3o G.(2020).Gonadal morphometry and histology data from B\u00edsaro pigs In: Paix\u00e3o G. (Ed.) Mendeley data.https:\/\/doi.org\/10.17632\/5t8v7r8mwm.1","DOI":"10.17632\/5t8v7r8mwm.1"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1590\/rbz4720170331"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.17221\/113\/2018-CJAS"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2019.05.010"},{"issue":"3","key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1017\/S0962728600030487","article-title":"A review of the welfare consequences of surgical castration in piglets and the evaluation of non\u2010surgical methods","volume":"15","author":"Prunier A.","year":"2006","journal-title":"Animal Welfare"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.07.030"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24748"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(93)90343-4"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(97)00239-2"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13827","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T08:15:22Z","timestamp":1693728922000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,14]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/rda.13827"],"URL":"https:\/\/doi.org\/10.1111\/rda.13827","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2020,10,14]]},"assertion":[{"value":"2020-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}