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The purpose of this study was to investigate the effects of UFMylation on lipopolysaccharide (LPS)\u2010induced inflammatory responses in immortalized goat endometrial epithelial cells (gEECs). Ubiquitin\u2010fold modifier conjugating enzyme 1 (UFM1) and DDRGK domain containing 1 (DDRGK1) were mainly localized in the luminal epithelium and glandular epithelium of mouse and goat endometrial tissues. The expression levels of UFM1, ubiquitin\u2010like modifier activating enzyme 5 (UBA5), UFM1 specific ligase 1 (UFL1) and DDRGK1, as key components of the UFMylation system, were significantly activated by 5\u00a0\u03bcg\/mL LPS\u2010induced inflammatory response in gEECs for 6\u00a0hr. Meanwhile, the expression levels of interleukin\u20106 (IL\u20106) were significantly upregulated, and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) was significantly down\u2010regulated after overexpression of UFM1 in gEECs. Additionally, we observed UFM1 and DDRGK1 were markedly increased on LPS\u2010stimulated mouse endometritis in vivo. In conclusion, the current study demonstrated that UFMylation was significantly activated by LPS and might be involved in regulating inflammatory response in gEECs.<\/jats:p>","DOI":"10.1111\/rda.13832","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T18:07:07Z","timestamp":1601316427000},"page":"1725-1734","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["UFMylation is associated with LPS\u2010induced inflammatory response in goat endometrial epithelial cells"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4033-6364","authenticated-orcid":false,"given":"Jinbang","family":"Xiao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University  Yangling China"},{"name":"Key Laboratory of Animal Biotechnology Ministry of Agriculture and Rural Affairs Northwest A&amp;F University  Yangling 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