{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T22:33:25Z","timestamp":1766788405264,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2013\u20103260"],"award-info":[{"award-number":["PICT 2013\u20103260"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["V11\/230"],"award-info":[{"award-number":["V11\/230"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the present study was to compare the endometrial gene expression of epidermal growth factor receptor (EGFR), nodal growth differentiation factor (NODAL), prostaglandin\u2010endoperoxide synthase 2 (PTGS2), oestrogen receptor 1 (ESR1) and progesterone receptor (PGR) in repeat breeder cows (RBC) and non\u2010RBC during diestrus. Endometrial samples were collected by cytobrush technique and stored in RNA stabilizing solution at \u221220\u00b0C until RT\u2010qPCR analysis. Differences in endometrial mRNA expression of selected genes were assessed by ANOVA and simple (r) and the partial correlations (rp) among selected genes were performed. Results demonstrated that mRNA expression of EGFR and NODAL were higher in RBC than in non\u2010RBC (3 and 25\u2010fold change, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01 and <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01, respectively), while the mRNA expression of PTGS2 was lower (1.56\u2010fold change, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). Although there were no differences detected in the mRNA expression of ESR1 and PGR, there was a positive correlation between the expression of ESR1 and EGFR (0.84, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and a negative correlation between PGR and PTGS2 (\u22120.49, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, the difference on the endometrial mRNA expression of the genes included in the study between RBC and non\u2010RBC indicates a deregulation of important mechanisms that are vital to establish a successful pregnancy. Thus, the present study provides useful insight as a base for future studies to elucidate the causes of RBC.<\/jats:p>","DOI":"10.1111\/rda.13841","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T02:28:47Z","timestamp":1602556127000},"page":"1660-1664","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Endometrial expression of key genes related to fertility in repeat breeder and non\u2010repeat breeder cows"],"prefix":"10.1111","volume":"55","author":[{"given":"Mar\u00eda","family":"Jaureguiberry","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Veterinarias Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA) Universidad Nacional de La Plata (FCV\u2010UNLP) La Plata Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Vanina","family":"Madoz","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA) Universidad Nacional de La Plata (FCV\u2010UNLP) La Plata Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvina","family":"Quintana","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"Facultad de Ciencias Exactas y Naturales Centro de Investigaci\u00f3n en Abejas Sociales Universidad Nacional de Mar del Plata Mar del Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maia","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"},{"name":"Estaci\u00f3n Experimental Agropecuaria Balcarce Instituto Nacional de Tecnolog\u00eda Agropecuaria Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes","family":"Buruc\u00faa","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas y Naturales Centro de Investigaci\u00f3n en Abejas Sociales Universidad Nacional de Mar del Plata Mar del Plata Argentina"},{"name":"Estaci\u00f3n Experimental Agropecuaria Balcarce Instituto Nacional de Tecnolog\u00eda Agropecuaria Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Tizzano","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda FCV\u2010UNLP La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Rabaglino","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramiro","family":"Rearte","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo \u00cdtalo","family":"Pecoraro","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda FCV\u2010UNLP La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9417-9560","authenticated-orcid":false,"given":"Rodolfo Luzbel","family":"de la Sota","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA) Universidad Nacional de La Plata (FCV\u2010UNLP) La Plata Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-012-1030-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-0088"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-1891-5-53"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01171.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.089979"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01317.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.085910"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20078"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2012-6269"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2004.01.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(01)00143-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400641200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0113"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.placenta.2010.12.004","article-title":"Elsevier trophoblast research award lecture: The multifaceted role of nodal signaling during mammalian reproduction","volume":"25","author":"Park C. B.","year":"2011","journal-title":"Placenta"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520295.2011.556666"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.04.005"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gau013"},{"key":"e_1_2_9_21_1","first-page":"328","article-title":"Expression of epidermal growth factor (EGF) and its receptor in bovine endometrium throughout the luteal phase: Effects of EGF on prostaglandin production in endometrial cells","volume":"12","author":"Takatsu K.","year":"2015","journal-title":"Animal Reproduction"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.12.013"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.01.016"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13841","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13841","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13841","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T09:28:03Z","timestamp":1693819683000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,27]]},"references-count":22,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1111\/rda.13841"],"URL":"https:\/\/doi.org\/10.1111\/rda.13841","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2020,10,27]]},"assertion":[{"value":"2020-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}