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The purpose of this study was to characterize the monthly variation in the steroid reproductive hormone metabolite levels (oestradiol, progesterone and testosterone) in this species and to test its possible association with a monthly pattern of faecal marking. By collecting fresh faecal samples in Montes do Invernadeiro Natural Park (Galicia, Northwest Spain) each month, we obtained a total of 110 samples belonging to 25 different individuals. We conducted enzyme immunoassays which allowed us to track the annual variation in reproductive hormone excretion patterns in wildcat scats. Furthermore, we also evaluated the possible relation between the faeces used as marks and the reproductive hormone levels. We found that oestradiol and progesterone metabolite levels exhibited a distinct pattern, both increasing during the breeding months. Oestradiol metabolite larger peaks were found during March and April, whereas the highest concentration of progesterone metabolites appeared in July. On the contrary, testosterone metabolite levels did not significantly change depending on the month. Moreover, we did not find any evidence that the faecal marking behaviour pattern was associated with reproductive hormone metabolite levels. It seems that other factors related to habitat and food resources could be more important in the performance of this behaviour.<\/jats:p>","DOI":"10.1111\/rda.13843","type":"journal-article","created":{"date-parts":[[2020,10,18]],"date-time":"2020-10-18T09:16:00Z","timestamp":1603012560000},"page":"1784-1793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Reproductive hormones monthly variation in free\u2010ranging European wildcats: Lack of association with faecal marking"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6892-0047","authenticated-orcid":false,"given":"Ana","family":"Pi\u00f1eiro","sequence":"first","affiliation":[{"name":"Escuela de Medicina Veterinaria Universidad Andr\u00e9s Bello, Republica 440 Santiago de Chile Chile"},{"name":"Unidad Zoolog\u00eda Departamento de Biolog\u00eda Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00aa Carmen","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Unidad Zoolog\u00eda Departamento de Biolog\u00eda Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Silv\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda (Fisiolog\u00eda Animal) Facultad de Veterinaria Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Illera","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda (Fisiolog\u00eda Animal) Facultad de Veterinaria Universidad Complutense de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Barja","sequence":"additional","affiliation":[{"name":"Unidad Zoolog\u00eda Departamento de Biolog\u00eda Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Centro de Investigaci\u00f3n en Biodiversidad y Cambio Global (CIBC\u2010UAM) Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,22]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"A dataset of the estradiol, fecal estradiol metabolite, progesterone, and fecal progesterone metabolite concentrations of female felids","author":"Andrews C. 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