{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T20:18:52Z","timestamp":1787602732792,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T00:00:00Z","timestamp":1607558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31860297"],"award-info":[{"award-number":["31860297"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Few studies exist on homozygous myostatin gene mutant (<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>) pigs, especially on their reproductive ability. We have previously shown that semen quality of homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>boars is comparable to that of wild type (WT). However, no data exist on the reproductive ability of heterozygous<jats:italic>MSTN<\/jats:italic>gene mutant (<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>) sows. The present study highlights showed that the heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>sows have delayed pubertal age than WT sows (255.80\u00a0\u00b1\u00a06.79 versus 191.10\u00a0\u00b1\u00a03.42, respectively). The number of services per pregnancy of heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>sows is significantly higher than that of WT sows (3.33\u00a0\u00b1\u00a00.43 versus 1.60\u00a0\u00b1\u00a00.25, respectively). Moreover, although heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>sows have natural reproduction ability, their litter size was significantly lower than that of WT sows (7.75\u00a0\u00b1\u00a00.44 versus 14.25\u00a0\u00b1\u00a00.60, respectively). Offsprings generated from heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>sow and homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>boar were genotyped with the PCR and sequencing method to detect myostatin mutation and to identify whether the piglets are homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>or heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>. The proportion of homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>piglets was significantly lower than that of heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>piglets (2.50\u00a0\u00b1\u00a00.35 versus 5.25\u00a0\u00b1\u00a00.60, respectively). Furthermore, none of the sows presented dystocia, and the phenotype of heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>piglets was normal. However, 10% homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>piglets died of dyspnoea within 2\u00a0hr after birth, 60% of homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>piglets showed large tongues, and 50% had umbilical hernias. In summary, this study for the first time reports the reproduction traits of heterozygous<jats:italic>MSTN<jats:sup>+\/<\/jats:sup><\/jats:italic><jats:sup>\u2212<\/jats:sup>sows crossbred with homozygous<jats:italic>MSTN<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>boars. This study will pave the way in a new direction for the breeding and development of super lean meat varieties in the future.<\/jats:p>","DOI":"10.1111\/rda.13845","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T18:43:56Z","timestamp":1603133036000},"page":"26-33","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Reproduction traits of heterozygous<i>myostatin<\/i>knockout sows crossbred with homozygous<i>myostatin<\/i>knockout boars"],"prefix":"10.1111","volume":"56","author":[{"given":"Sheng\u2010zhong","family":"Han","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou\u2010Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo\u2010Jin","family":"Paek","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hak\u2010Myong","family":"Choe","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0322-3560","authenticated-orcid":false,"given":"Xi\u2010Jun","family":"Yin","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao\u2010Hu","family":"Quan","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering Yanbian University Yanji China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/AR9951493"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1989.674902x"},{"issue":"10","key":"e_1_2_10_4_1","first-page":"1065","article-title":"Evaluation of the effect of umbilical hernias on play behaviors in growing pigs","volume":"58","author":"Atkinson M.","year":"2017","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2017.06.017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.06.014"},{"key":"e_1_2_10_7_1","volume-title":"Welfare and belgian blue cattle","author":"Bassett A.","year":"2009"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2004.01229.x"},{"key":"e_1_2_10_9_1","first-page":"575","volume-title":"Analysis of Reproduction Problems in Double Muscled Females. 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