{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:34:59Z","timestamp":1779917699538,"version":"3.53.1"},"reference-count":66,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002378","name":"Faculty of Veterinary Medicine, Cairo University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002378","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Melatonin is known to protect sperm against freezing\u2010inflicted damage in different domestic species. The aim of the study was to evaluate the effect of supplementation of semen extender with melatonin on the quality and DNA integrity of cooled and frozen\/thawed rabbit spermatozoa. We also investigated whether the addition of melatonin to the semen extender could improve the fertility of rabbit does artificially inseminated with frozen\/thawed semen. Semen samples collected from eight rabbit bucks were pooled and then diluted in INRA\u201082 supplemented either with (0.5, 1.0 or 1.5\u00a0mM) or without (0.0\u00a0mM) melatonin. Diluted semen was cooled at 5\u00b0C for 24\u00a0hr. For cryopreservation and based on the first experiment's best result, semen samples were diluted in INRA\u201082 in the presence or absence of 1.0\u00a0mM melatonin and then frozen in 0.25\u00a0ml straws. Following cooling or thawing, sperm quality and DNA integrity were evaluated. Furthermore, the fertility of frozen\/thawed semen was investigated after artificial insemination. Supplementation of semen extender with 1.0\u00a0mM melatonin improved (<jats:italic>p\u00a0&lt;\u00a0<\/jats:italic>.05) motility, viability, membrane and acrosome integrities in cooled semen compared with other groups. Sperm quality and DNA integrity were higher (<jats:italic>p\u00a0&lt;\u00a0<\/jats:italic>.05) in frozen\/thawed semen diluted in 1.0\u00a0mM melatonin\u2010supplemented extender than in the control group. Conception and birth rates were higher in does inseminated with 1.0\u00a0mM melatonin treated semen compared with the controls. In conclusion, supplementation of semen extender with 1.0\u00a0mM melatonin improved the quality of cooled and frozen\/thawed rabbit spermatozoa. Melatonin can preserve DNA integrity and enhance the fertility of frozen\/thawed rabbit spermatozoa.<\/jats:p>","DOI":"10.1111\/rda.13853","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T07:22:46Z","timestamp":1604474566000},"page":"103-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Melatonin can improve viability and functional integrity of cooled and frozen\/thawed rabbit spermatozoa"],"prefix":"10.1111","volume":"56","author":[{"given":"Aya M.","family":"Fadl","sequence":"first","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel Raouf M.","family":"Ghallab","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa M.","family":"Abou\u2010Ahmed","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4397-9688","authenticated-orcid":false,"given":"Adel R.","family":"Moawad","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00559.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05328.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950659"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0810459"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.5.1037"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-1188-1_38"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1992.tb00306.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2013.03.016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2005.00624.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2003.10.068"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-3-67"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2016.1286"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-125"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2008.04.003"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2009.00700.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-8-59"},{"issue":"2","key":"e_1_2_9_18_1","first-page":"115","article-title":"Effect of melatonin supplementation on cryopreserved sperm quality in mouse","volume":"37","author":"Chen X. 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