{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:58:56Z","timestamp":1753275536189,"version":"3.40.5"},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/21452\u20105"],"award-info":[{"award-number":["2016\/21452\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study compared the quality of sperm collected by artificial vagina or pharmacologically induced ejaculation from a 10\u2010year\u2010old thoroughbred stallion with seminal vesiculitis. The pharmacological protocol involved intravenous administration of detomidine (0.01\u00a0mg\/kg) and oxytocin (20\u00a0IU) and successfully induced ejaculation in all attempts of semen collection. Sperm motility, plasma membrane and acrosome integrity (PMAI), reactive oxygen species (ROS) levels, polymorphonuclear neutrophil (PMN) percentage, and bacterial profiles of fresh and cooled semen (5\u00b0C for 24\u00a0hr) were evaluated. Semen obtained by the pharmacological method presented reduced seminal volume, decreased PMN percentage and superior sperm motility in cooled samples. Moreover, higher PMAI and lower ROS levels were observed in semen collected by the pharmacological method. Therefore, pharmacologically induced ejaculation is an alternative to obtain semen with minimal contamination and with sperm of superior quality and longevity from stallions with seminal vesiculitis.<\/jats:p>","DOI":"10.1111\/rda.13860","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T06:59:58Z","timestamp":1605164398000},"page":"1808-1811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of semen collected by pharmacologically induced ejaculation from a stallion with seminal vesiculitis"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5249-1313","authenticated-orcid":false,"given":"Tha\u00eds Mendes Sanches","family":"Cavalero","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7526-7760","authenticated-orcid":false,"given":"Lorenzo Garrido Teixeira M.","family":"Segabinazzi","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0726-7934","authenticated-orcid":false,"given":"Ver\u00f4nica Flores da Cunha","family":"Scheeren","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila P.","family":"Freitas\u2010Dell'Aqua","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederico Ozanam","family":"Papa","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00710-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.08.024"},{"key":"e_1_2_9_4_1","first-page":"941","article-title":"Flow cytometric analysis of fertile and subfertile frozen stallion spermatozoa","volume":"9","author":"Freitas\u2010Dell'Aqua C. 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