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We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5\u00a0days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3\u00a0days, while sperm motility at pH 8.2 decreased significantly after 2\u00a0days. Non\u2010capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration\u2010dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility\u2010related protein expression after 2\u00a0days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2\u00a0days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.<\/jats:p>","DOI":"10.1111\/rda.13870","type":"journal-article","created":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T17:51:37Z","timestamp":1606672297000},"page":"333-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New technique of sex preselection for increasing female ratio in boar sperm model"],"prefix":"10.1111","volume":"56","author":[{"given":"Yoo\u2010Jin","family":"Park","sequence":"first","affiliation":[{"name":"Department of Animal Science &amp; Technology and BET Research Institute Chung\u2010Ang University Anseong Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki\u2010Jin","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Animal Science &amp; Technology and BET Research Institute Chung\u2010Ang University Anseong 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