{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:50:08Z","timestamp":1777524608049,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex\u2010evacuation zone. Wildlife within the alert zone have been exposed to low\u2010dose\u2010rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex\u2010evacuation zone of the FDNPP accident. The radioactivity of caesium\u2010137 (<jats:sup>137<\/jats:sup>Cs) was measured by gamma\u2010ray spectrometry, and the measured radioactivity concentration was 300\u20136,630\u00a0Bq\/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze\u2010thawing sperm penetration ability was confirmed using the interspecific zona pellucida\u2010free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons.<\/jats:p>","DOI":"10.1111\/rda.13887","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T08:20:20Z","timestamp":1609230020000},"page":"484-497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (<i>Procyon lotor<\/i>)"],"prefix":"10.1111","volume":"56","author":[{"given":"Kazuki","family":"Komatsu","sequence":"first","affiliation":[{"name":"Graduate School of Science and Technology Niigata University Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsugumi","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology Niigata University Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Murata","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology Niigata University Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0133-2459","authenticated-orcid":false,"given":"Hideaki","family":"Yamashiro","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology Niigata University Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie Swee Ting","family":"Goh","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences Hirosaki University Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences Hirosaki University Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Fujishima","sequence":"additional","affiliation":[{"name":"Department of Radiation Biology Tohoku University School of Medicine Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takumi","family":"Ono","sequence":"additional","affiliation":[{"name":"Graduate School of Science Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kino","sequence":"additional","affiliation":[{"name":"Graduate School of Science Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinaka","family":"Simizu","sequence":"additional","affiliation":[{"name":"Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Shinoda","sequence":"additional","affiliation":[{"name":"Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Ariyoshi","sequence":"additional","affiliation":[{"name":"Integrated Center for Science and Humanities Fukushima Medical University Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Kasai","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences Hirosaki University Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"International Research Institute of Disaster Science Tohoku University Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Palmerini","sequence":"additional","affiliation":[{"name":"Department of Life, Health and Environmental Sciences University of L'Aquila L'Aquila Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Belli","sequence":"additional","affiliation":[{"name":"Department of Life, Health and Environmental Sciences University of L'Aquila L'Aquila Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Macchiarelli","sequence":"additional","affiliation":[{"name":"Department of Life, Health and Environmental Sciences University of L'Aquila L'Aquila Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshitaka","family":"Oka","sequence":"additional","affiliation":[{"name":"Sector of Nuclear Science Research Japan Atomic Energy Agency Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"RIKEN Center for Advanced Intelligence Project, Pathology Informatics Team Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuaki A.","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akifumi","family":"Nakata","sequence":"additional","affiliation":[{"name":"Department of Life Science, Faculty of Pharmaceutical Sciences Hokkaido University of Science Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomisato","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Risk Analysis and Biodosimetry, Institute of Radiation Emergency Medicine Hirosaki University Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs021"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00411-018-0743-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.369"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez011"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00561-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.12.031"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12490"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12301"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054312"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-038-0_10"},{"key":"e_1_2_9_12_1","first-page":"125","article-title":"Present status of invasive alien raccoon and its impact in Japan","volume":"8","author":"Ikeda T.","year":"2004","journal-title":"Global Environmental Research"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106288"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-016-0858-6"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.16069"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-8218-5_5"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24077"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvrad.2017.12.005"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1050099"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13573"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.08.010"},{"key":"e_1_2_9_22_1","first-page":"1","volume-title":"Ionizing and non\u2010ionizing radiation","author":"Ohdaira T.","year":"2019"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0351"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2013-122"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2017.09.008"},{"key":"e_1_2_9_26_1","unstructured":"R Core Team(2020).R: A language and environment for statistical computing. (Version 3.6.3).Vienna Austria:R Foundation for Statistical Computing. Retrieved from http:\/\/www.r\u2010project.org\/"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.021"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1515\/mamm.2000.64.4.369"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.21900\/j.inhs.v31.146"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-8218-5_10"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199418000138"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jrr\/rrv069"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR14234.1"},{"key":"e_1_2_9_34_1","unstructured":"Thieurmel B &Elmarhraoui A(2019).suncalc: Compute Sun Position Sunlight Phases Moon Position and Lunar Phase. (R package version 0.5.0). Retrieved from https:\/\/CRAN.R\u2010project.orgpackage=suncalc"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2014-011"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155069"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35104-0"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.05.004"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02850"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jrr\/rrv070"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.5772\/58629"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod15.4.471"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13887","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13887","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T03:28:08Z","timestamp":1693538888000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,18]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/rda.13887"],"URL":"https:\/\/doi.org\/10.1111\/rda.13887","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,18]]},"assertion":[{"value":"2020-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}