{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:26:11Z","timestamp":1788611171141,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T00:00:00Z","timestamp":1618617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The differential proteins associated with plasma membrane of spermatozoa are less known, identification of which shall help overcome limitations of currently used methods of sperm sexing, considered as a high priority for livestock sector of many countries. This study has reported plasma membrane proteomics of unsorted spermatozoa and differential expression of plasma membrane\u2010associated proteins between X\u2010 and Y\u2010chromosome bearing spermatozoa of indicus cattle (<jats:italic>Bos indicus<\/jats:italic>). Isolation of plasma membrane fraction using percoll gradient, relatively a rapid method, from bovine spermatozoa has been reported to enrich isolation of plasma membrane proteins. Significant enrichment for plasma membrane\u2010associated proteins was observed in plasma membrane fraction (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) as compared to the total cell lysate using LC\u2010MS\/MS. Furthermore, these experiments were conducted in flow cytometry sorted, sexed\u2010semen samples. Thirteen proteins were identified as differentially abundant between X\u2010 and Y\u2010sorted spermatozoa. Among these, two proteins were downregulated in Y\u2010sorted spermatozoa compared to the X\u2010sorted spermatozoa (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), while four and seven proteins could be noted in X\u2010 and Y\u2010sorted spermatozoa, respectively. Proteins that are presumed to support sperm capacitation and sperm migration velocity were found to be abundant in Y\u2010sorted spermatozoa while those associated with structural molecule activity were identified as abundant in X\u2010sorted spermatozoa in the present study. Our study provides better insight into the plasma membrane proteomics of spermatozoa of indicus cattle and furnishes data that might aid in design and development of alternate and open technology for sex\u2010sorting of semen.<\/jats:p>","DOI":"10.1111\/rda.13936","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:55:12Z","timestamp":1617843312000},"page":"928-935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Differential proteins associated with plasma membrane in X\u2010 and\/or Y\u2010chromosome bearing spermatozoa in indicus cattle"],"prefix":"10.1111","volume":"56","author":[{"given":"Rongala","family":"Laxmivandana","sequence":"first","affiliation":[{"name":"ICAR\u2010Indian Institute of Agricultural Biotechnology Ranchi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chhaya","family":"Patole","sequence":"additional","affiliation":[{"name":"Bangalore Life Science Cluster Bengaluru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilak Raj","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Institute of Agricultural Biotechnology Ranchi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewal Krishan","family":"Sharma","sequence":"additional","affiliation":[{"name":"ICAR\u2010 Indian Institute of Natural Resins and Gums Ranchi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1873-2221","authenticated-orcid":false,"given":"Soumen","family":"Naskar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Institute of Agricultural Biotechnology Ranchi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh037"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.14737\/journal.aavs\/2016\/4.12.613.618"},{"issue":"12","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.56093\/ijans.v83i12.35801","article-title":"Flow cytometric sorting of Sahiwal bulls semen coupled with artificial insemination at organized herd","volume":"83","author":"Biswas J.","year":"2013","journal-title":"Indian Journal of Animal Sciences"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0334-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.07.004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900521"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3MB70306A"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-11-0228"},{"issue":"1","key":"e_1_2_9_11_1","first-page":"1","article-title":"Advances in sperm sexing in bovines","volume":"21","author":"Hanuman P. 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