{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:37Z","timestamp":1787016097504,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005363","name":"Universidad de Buenos Aires","doi-asserted-by":"publisher","award":["UBACyT 20020190200084BA"],"award-info":[{"award-number":["UBACyT 20020190200084BA"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Semen cryopreservation is not available for massive use in South American Camelids (SACs) due to the lack of an efficient protocol and the low pregnancy rates obtained with artificial insemination (AI). The use of a single cryoprotectant (CP) is commonly used in SACs frozen semen. The objective of the study was to evaluate the combined cryoprotective capacity of two permeable CPs at different stages of the cryopreservation protocol in llama semen. Sixteen ejaculates from 4 llama males were analysed, and sperm quality was assayed in raw semen, at 5\u00b0C, after equilibration of samples with the CPs and when samples were thawed. The following CPs and combination were used: 6% glycerol (GL), 6% dimethylformamide (DMF) and the combination of both CPs: 3% GL and 3% DMF. A Kruskal\u2013Wallis test and an experimental factorial design, considering one factor with four levels (raw semen, 6% GL, 6% DMF and GL\/DMF), were used. Total sperm motility and live sperm with intact acrosomes remained unchanged after equilibration of samples (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a0.05). A significant decrease in the percentage of functional membrane, motile and live sperm with intact acrosomes was observed when samples were thawed (GL, DMF and GL\/DMF). Nevertheless, the cryopreservation protocols used preserved sperm DNA quality; thus, sperm chromatin condensation and DNA fragmentation were unaffected (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a0.05) when GL, DMF and GL\/DMF were used. To conclude, no superiority was found between the use of a single or a combination of permeable cryoprotectants to freeze llama semen.\n                  <\/jats:p>","DOI":"10.1111\/rda.13937","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T05:24:50Z","timestamp":1617859490000},"page":"958-964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Cryopreservation of llama semen using a combination of permeable cryoprotectants"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4561-9005","authenticated-orcid":false,"given":"Fernanda Gabriela","family":"Fumuso","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires Facultad de Ciencias Veterinarias Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) C\u00e1tedra de Teriogenolog\u00eda  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Luc\u00eda","family":"Bertuzzi","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires Facultad de Ciencias Veterinarias Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) C\u00e1tedra de Teriogenolog\u00eda  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Vel\u00e1squez Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires Facultad de Ciencias Veterinarias Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) C\u00e1tedra de Teriogenolog\u00eda  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo H.","family":"Miragaya","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires Facultad de Ciencias Veterinarias Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) C\u00e1tedra de Teriogenolog\u00eda  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-0580","authenticated-orcid":false,"given":"Maria Ignacia","family":"Carretero","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires Facultad de Ciencias Veterinarias Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA) C\u00e1tedra de Teriogenolog\u00eda  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,20]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Equilibration time during freezing of llama semen","author":"Alarc\u00f3n V.","year":"2004"},{"key":"e_1_2_9_3_1","first-page":"15","article-title":"Influencia de la criopreservaci\u00f3n sobre la movilidad, viabilidad y fertilidad de espermatozoides de llama (Lama glama)","volume":"52","author":"Aller J. F.","year":"2003","journal-title":"Archives of Zootecnia"},{"key":"e_1_2_9_4_1","first-page":"686137","article-title":"Impacts of oxidative stress and antioxidants on semen functions","volume":"7","author":"Bansal A. K.","year":"2011","journal-title":"Veterinary Medicine International"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.11.008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00158-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.15381\/rivep.v26i3.11185"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.06.014"},{"key":"e_1_2_9_9_1","first-page":"55","article-title":"Evaluaci\u00f3n del ADN esperm\u00e1tico de llamas utilizando azul de toluidina","volume":"11","author":"Carretero M. 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