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Aucubin (AU) has been proven to exhibit highly effective anti\u2010inflammatory activity, but its ability to protect against endometritis in dairy cows remains unclear. Therefore, the goal of the present study was to evaluate the protective effect of AU on the LPS\u2010induced inflammatory response of bovine endometrial epithelial cells (BEECs). After pre\u2010treating BEECs with AU (10, 20 and 50\u00a0\u03bcM) for 6\u00a0hr, the cells were stimulated with LPS for 3\u00a0hr. Subsequently, BEECs apoptosis was analysed by flow cytometry, the expression of pro\u2010inflammatory cytokine mRNA was detected by qRT\u2010PCR, and changes in NF\u2010\u03baB and Keap1\/Nrf2 signalling were analysed by western blotting and immunofluorescence analyses. The results showed that AU can reduce TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, COX\u20102 and iNOS mRNA expression in BEECs and reduce cell apoptosis. Furthermore, AU significantly reduced the level of NF\u2010\u03baB p65 and I\u03baB phosphorylation and inhibited the nuclear translocation of NF\u2010\u03baB p65. AU also activated the Keap1\/Nrf2 pathway, promoting the nuclear transfer of Nrf2 and increasing Keap1, Nrf2, HO\u20101 and NQO1 mRNA and protein levels. Taken together, these results indicate that AU ameliorates the LPS\u2010induced inflammatory response by inhibiting NF\u2010\u03baB and activating the Keap1\/Nrf2 signalling pathway, which has a protective effect on BEECs.<\/jats:p>","DOI":"10.1111\/rda.13939","type":"journal-article","created":{"date-parts":[[2021,4,18]],"date-time":"2021-04-18T18:21:05Z","timestamp":1618770065000},"page":"972-982","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Aucubin ameliorates the LPS\u2010induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\u2010\u03baB and activating the Keap1\/Nrf2 signalling pathway"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7295-7229","authenticated-orcid":false,"given":"Feng","family":"Gao","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huatao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanni","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenru","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongfeng","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiqiang","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Qingdao Agricultural University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,27]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"585","article-title":"(2017) Nrf2 signaling pathway: Pivotal roles in inflammation","volume":"2","author":"Ahmed S. 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