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This study was aimed at determining the impacts of 3,4\u2010dihydroxyphenyl glycol (DHPG) on the spermatozoa of the canine undergoing freeze\u2010thawing process. In this study, 24 ejaculates were obtained from three mixed\u2010breed dogs and were diluted in a Tris\u2010based extender. The diluted semen was divided into aliquots for supplementation of 10, 30, 50 and 70\u00a0\u00b5g\/ml of DHPG, control (without antioxidant) and control sham (DMSO). After being extended, the semen was equilibrated at a temperature of 4\u00b0C and then transferred to the straws and kept 4\u00a0cm above the liquid nitrogen for 20\u00a0min and was finally immersed in the liquid nitrogen. They were cryopreserved for seven days; then, sperm parameters including sperm motility evaluation, motility characteristics, viability, DNA and plasma membrane integrity, total antioxidant capacity (TAC), reduced glutathione content (GSH), antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT] and glutathione peroxidase [GPx]) activity malondialdehyde (MDA) levels were evaluated. This study showed that spermatozoa cryopreservation with 50, 30 and 70\u00a0\u00b5g\/ml of DHPG concentrations had better progressive motility, Curvilinear Velocity, Linearity, viability, intact plasma membrane and the levels of TAC, GPx and GSH were higher than the control group. The 50, 30 and 70\u00a0\u00b5g\/ml of DHPG concentrations led to the significant decrease of DNA damage compared to the control group. Total motility, average path velocity, straight\u2010line velocity and CAT activity were significantly improved in 30 and 50\u00a0\u00b5g\/ml of DHPG concentrations, compared to the control group. Also, the 50 and 30\u00a0\u00b5g\/ml of DHPG concentrations, decreased MDA levels compared to the other groups, significantly. In conclusion, our study showed that the addition 50\u00a0\u00b5g\/ml of DHPG to the canine semen extender improved the semen characteristics and oxidative markers in the cryopreservation process.<\/jats:p>","DOI":"10.1111\/rda.13944","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T08:49:35Z","timestamp":1619599775000},"page":"1004-1014","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Antioxidant effects of supplementation of 3,4\u2010dihydroxyphenyl glycol on sperm parameters and oxidative markers following cryopreservation in canine semen"],"prefix":"10.1111","volume":"56","author":[{"given":"Nastaran","family":"Shakouri","sequence":"first","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Urmia University Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1591-2198","authenticated-orcid":false,"given":"Ali","family":"Soleimanzadeh","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine Urmia University Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaleh","family":"Rakhshanpour","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Clinical Pathology Faculty of Veterinary Medicine Urmia University Urmia Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2955-8599","authenticated-orcid":false,"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Selcuk University Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-012-2675-x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13098"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.05136"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03089"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"key":"e_1_2_10_7_1","first-page":"3","article-title":"Adding olive oil to rooster semen diluents for improving semen quality and storage ability during liquid storage","volume":"2","author":"Al\u2010Daraji H. 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