{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:37:40Z","timestamp":1790779060914,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aglepristone, a competitive progesterone antagonist, is successfully used in various progesterone\u2010dependent conditions. This study investigated uterine histomorphometric analysis, and expressions of the oestrogen \u03b1 receptor (ER\u03b1) and progesterone receptor (PR) in uteri of bitches following the single dose of aglepristone treatment. Twelve client\u2010owned healthy diestrous bitches were used in the study. The single dose of aglepristone (Alizine<jats:sup>\u00ae<\/jats:sup>, 10\u00a0mg\/kg) was injected subcutaneously 5\u00a0days before ovariohysterectomy in the treatment group (<jats:italic>n<\/jats:italic>\u00a0=\u00a06); bitches without treatment served as a control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a06). Uteri were collected for histomorphometric analysis, ER\u03b1 and PR gene, and protein expressions studies. The mRNA expressions of ER\u03b1 and PR were determined by RT\u2010qPCR. Immunohistochemical analysis was used to evaluate the ER\u03b1 and PR protein expressions using an H\u2010score in five parts of the uterus. The results demonstrated glandular epithelium height significantly decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and ER\u03b1 mRNA increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) in treated dogs. Of the treated bitches, lower expression levels of ER\u03b1 were observed in the luminal epithelium, crypt and glandular epithelium, with higher expression in the endometrial stroma and myometrium (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05); however, PR expression decreased in the luminal epithelium, crypt and glandular epithelium (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). In conclusion, reduction of the uterine glandular epithelium and ER\u03b1 mRNA upregulation together with changes in ER\u03b1 and PR expressions were observed in the treated bitches. However, changes in uterine ER\u03b1 and PR expressions in the treated bitches depended on tissue layers. The treatment had no effect on serum oestradiol and progesterone levels.<\/jats:p>","DOI":"10.1111\/rda.13945","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T18:49:22Z","timestamp":1619722162000},"page":"1015-1023","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Molecular studies on oestrogen \u03b1 and progesterone receptors and histomorphometric analysis of canine uteri following aglepristone treatment"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2458-1754","authenticated-orcid":false,"given":"Chunsumon","family":"Limmanont","sequence":"first","affiliation":[{"name":"Center for Advanced Studies in Agriculture and Food KU Institute for Advanced Studies Kasetsart University (CASAF, NRU\u2010KU)  Bangkok Thailand"},{"name":"Department of Companion Animal Clinical Sciences Faculty of Veterinary Medicine Kasetsart University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeda","family":"Lertwatcharasarakul","sequence":"additional","affiliation":[{"name":"Department of Pathology Faculty of Veterinary Medicine Kasetsart University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suppawiwat","family":"Ponglowhapan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction Research Unit of Obstetrics and Reproduction in Animals Faculty of Veterinary Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2578-6121","authenticated-orcid":false,"given":"Kaitkanoke","family":"Sirinarumitr","sequence":"additional","affiliation":[{"name":"Center for Advanced Studies in Agriculture and Food KU Institute for Advanced Studies Kasetsart University (CASAF, NRU\u2010KU)  Bangkok Thailand"},{"name":"Department of Companion Animal Clinical Sciences Faculty of Veterinary Medicine Kasetsart University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Virbac Animal Health(2010). 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