{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T06:54:38Z","timestamp":1771916078651,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["112O032"],"award-info":[{"award-number":["112O032"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Laparoscopic artificial insemination technique (LAI) is described to overcome reduced fertility problems in sheep artificial insemination (AI) programmes with frozen semen. Later on, this technology was modified for endangered non\u2010domestic cats to deposit low quality or reduced number of sperm cells hardly obtained by electro\u2010ejaculation into the oviduct. This technique by passes the complex structure of cervix and efficiently transfers the sperm cells to the point of fertilization. In recent years, rabbits are becoming popular transgenic animal models producing various therapeutic and commercial products, as well as being experimental animals for disease models. The worldwide transportation of frozen semen and re\u2010establishment of transgenic lines using AI technology has become a common practice. Therefore, this study was designed to describe a laparoscopic intrauterine insemination technique, which might assist in conceiving the animals with limited number of sperm cells. The female rabbits were laparoscopically (<jats:italic>n<\/jats:italic>\u00a0=\u00a022) or vaginally (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) inseminated with frozen\u2013thawed semen samples containing approximately 10\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> motile sperm. The laparoscopic insemination technique provided higher pregnancy rate (45.5%) than vaginal insemination technique (7.7%) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, the described laparoscopic AI might be a new alternative technique, thus enabling limited or low\u2010quality frozen sperm samples to establish pregnancy in rabbits.<\/jats:p>","DOI":"10.1111\/rda.13949","type":"journal-article","created":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T13:25:50Z","timestamp":1620566750000},"page":"1059-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative efficiency of novel laparoscopic and routine vaginal inseminations with cryopreserved semen in rabbits"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3046-846X","authenticated-orcid":false,"given":"Niyazi","family":"K\u00fc\u00e7\u00fck","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination University of Ayd\u0131n Adnan Menderes  Ayd\u0131n Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U\u011fur","family":"U\u00e7an","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination University of Ayd\u0131n Adnan Menderes  Ayd\u0131n Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1819-1522","authenticated-orcid":false,"given":"Sanan","family":"Raza","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination University of Ayd\u0131n Adnan Menderes  Ayd\u0131n Turkey"},{"name":"Department of Animal Reproduction University of Veterinary &amp; Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9807-810X","authenticated-orcid":false,"given":"G\u00fcne\u015f","family":"Erdo\u011fan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Veterinary Obstetrics and Gynecology University of Ayd\u0131n Adnan Menderes  Ayd\u0131n Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2019-196X","authenticated-orcid":false,"given":"Melih","family":"Aksoy","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination University of Ayd\u0131n Adnan Menderes  Ayd\u0131n Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.02.014"},{"key":"e_1_2_10_3_1","unstructured":"Alvari\u00f1o J. Lopez F. Del Arco J. Bueno A. &Torres R.(1996).Effect of semen concentration on rabbit artificial insemination with fresh or 24 hours stored semen. Paper presented at the Proceedings of the Sixth World Rabbit Congress Toulouse France."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01485010290099255"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.07.032"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0990053"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/laban0405-61"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025816809372"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.17221\/6980-VETMED"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.07.021"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(99)00051-2"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS19750013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1005"},{"issue":"5","key":"e_1_2_10_15_1","first-page":"1249","article-title":"Artificial insemination of rabbits","volume":"25","author":"Dimitrova I.","year":"2009","journal-title":"Biotechnology in Animal Husbandry"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.09.005"},{"issue":"2","key":"e_1_2_10_17_1","first-page":"29","article-title":"Estimation du taux d'ovulation par laparoscopie chez la lapine: Effet de la couleur de la vulve et de l'\u00e9tat physiologique","volume":"5","author":"Forcada F.","year":"1989","journal-title":"Cuni Sci"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0380097"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(05)80017-X"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.29.3.445"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/691830"},{"issue":"2","key":"e_1_2_10_22_1","first-page":"337","article-title":"Tav\u015fanlarda genel anestezi","volume":"11","author":"Karabulut E.","year":"1997","journal-title":"F\u0131rat \u00dcniversitesi Sa\u011fl\u0131k Bilimleri Dergisi"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1982.tb02737.x"},{"issue":"2","key":"e_1_2_10_24_1","first-page":"201","article-title":"Reproductive performance of primiparous and multiparous Saanen goats after laparoscopic intrauterine insemination: A field study","volume":"36","author":"Kulaksiz R.","year":"2012","journal-title":"Turkish Journal of Veterinary and Animal Sciences"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.56.167"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.4995\/wrs.2012.1080"},{"issue":"3","key":"e_1_2_10_27_1","first-page":"76","article-title":"Intrauterine insemination with frozen\u2010thawed semen in Creole goats, synchronized in oestrous during the non\u2010breeding season","volume":"1","author":"Martinez R.","year":"2007","journal-title":"Research Journal of Animal Science"},{"issue":"2","key":"e_1_2_10_28_1","first-page":"60","article-title":"Comparison of different evaluation chambers for analysis of rabbit spermatozoa motility parameters using CASA system","volume":"41","author":"Massanyi P.","year":"2008","journal-title":"Slovak Journal of Animal Science"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.c5979"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2005.00619.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(05)80022-3"},{"key":"e_1_2_10_32_1","unstructured":"National Research Council. (2010).Guide for the care and use of laboratory animals."},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.150.10.299"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.156.12.372"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2019.09.008"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.07.041"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.2527\/af.2013-0030"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.5455\/ajvs.235400"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1999.tb02519.x"},{"key":"e_1_2_10_40_1","first-page":"1","volume-title":"Advances in Animal Biotechnology","author":"Schook L.","year":"2014"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)00067-5"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0240111"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12069"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18350"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2508.2002.00551.x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004640000256"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(96)01628-4"},{"issue":"2","key":"e_1_2_10_48_1","first-page":"95","article-title":"Early pregnancy diagnosis in the rabbit by real time ultrasonography","volume":"7","author":"Ypsilantis P.","year":"1999","journal-title":"World Rabbit Science"},{"key":"e_1_2_10_49_1","first-page":"10","article-title":"The applications of transgenic rabbits in agriculture and biomedicine","volume":"12","author":"Zabetian H. M.","year":"2011","journal-title":"Journal of Animal and Veterinary Advances"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13949","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13949","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13949","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T20:25:12Z","timestamp":1693340712000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,22]]},"references-count":48,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/rda.13949"],"URL":"https:\/\/doi.org\/10.1111\/rda.13949","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,22]]},"assertion":[{"value":"2021-04-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}