{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:11:17Z","timestamp":1783595477534,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,5,29]],"date-time":"2021-05-29T00:00:00Z","timestamp":1622246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to study the characteristics and subpopulations of spermatozoa from bulls with low and high reproductive performance based on pregnancy rates. Based on historical records of pregnancy rate from four farms, 24 bulls were selected. Two groups were established, with low pregnancy rates (<jats:italic>n<\/jats:italic>\u00a0=\u00a012; LOW), including bulls that presented pregnancy rates &lt;52.27% (33.33% to 51.81%); and a group with high pregnancy rates (<jats:italic>n<\/jats:italic>\u00a0=\u00a012; HIGH), with pregnancy rates &gt;52.27% (52.27% to 69.64%), after fixed\u2010time artificial insemination (FTAI). The thawed sperm straws were analysed to sperm kinetics, morphology, plasma membrane integrity and sperm subpopulations. The LOW group exhibited a higher proportion of static cells (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In contrast, the HIGH group showed greater percentages for membrane integrity and total and progressive motility, and cells with fast and medium velocity (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In the cluster procedures, four sperm subpopulations were established. The low\u2010fertility bulls presented the highest percentage of subpopulation 2 (41.46%), characterized by slow and progressive spermatozoa. The high\u2010fertility bulls exhibited the highest percentage of subpopulation 3 (37.17%), characterized by fast and nonlinear spermatozoa. Results from this study indicated that bulls with greater percentages of fast and nonlinear spermatozoa seem to have greater fertilization capacity and the subpopulations analysis can be considered a tool to identify ejaculates with high fertility.<\/jats:p>","DOI":"10.1111\/rda.13955","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T08:03:43Z","timestamp":1621497823000},"page":"1117-1127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Sperm subpopulations influence the pregnancy rates in cattle"],"prefix":"10.1111","volume":"56","author":[{"given":"Myrian Megumy Tsunokawa","family":"Hidalgo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida (LARAA) Universidade Estadual de Londrina (UEL)  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Beatriz Marques de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida (LARAA) Universidade Estadual de Londrina (UEL)  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Lucas Zito de","family":"Moraes","sequence":"additional","affiliation":[{"name":"M\u00e9dico Veterin\u00e1rio Aut\u00f4nomo  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Yudi Palhaci","family":"Marubayashi","sequence":"additional","affiliation":[{"name":"Departamento de P\u00f3s\u2010gradua\u00e7\u00e3o em Agronomia Universidade Estadual de Londrina (UEL)  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana Ferreira de","family":"Souza","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade Estadual Paulista (UNESP)  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thales Ricardo Rigo","family":"Barreiros","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia da Reprodu\u00e7\u00e3o Animal Universidade Estadual do Norte do Paran\u00e1 (UENP)  Bandeirantes Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8416-2450","authenticated-orcid":false,"given":"Maria Isabel Mello","family":"Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Andrologia e Reprodu\u00e7\u00e3o Animal Assistida (LARAA) Universidade Estadual de Londrina (UEL)  Londrina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.09.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2008.11.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2018.03.005"},{"key":"e_1_2_9_5_1","volume-title":"Evolu\u00e7\u00e3o e perspectivas da insemina\u00e7\u00e3o artificial em bovinos","author":"Baruselli P. S.","year":"2019"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.10.001"},{"key":"e_1_2_9_7_1","volume-title":"Manual para exame androl\u00f3gico e avalia\u00e7\u00e3o de s\u00eamen animal","author":"Col\u00e9gio Brasileiro de Reprodu\u00e7\u00e3o Animal (CBRA)","year":"2013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.03.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.22167\/r.ipecege.2018.1.28"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2017.07.002"},{"key":"e_1_2_9_11_1","first-page":"142","volume-title":"Andrology Special Interest Group. In Guidelines on the application of CASA technology in the analysis of spermatozoa","author":"European Society of Human Reproduction and Embryology (ESHRE)","year":"1998"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.01.033"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.05.013"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.056"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod34.1.127"},{"issue":"6","key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"871","DOI":"10.4103\/1008-682X.187583","article-title":"Normozoospermic versus teratozoospermic domestic cats: Differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation","volume":"18","author":"Guti\u00e9rrez\u2010Reinoso M.","year":"2016","journal-title":"Asian Journal of Andrology"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0797"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD07037"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD17476"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00134"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2017-083"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106329"},{"issue":"39","key":"e_1_2_9_23_1","first-page":"29","article-title":"Bull semen evaluation post\u2010thaw and relation of semen characteristics to bull's fertility","volume":"17","author":"Janu\u0161kauskas A.","year":"2002","journal-title":"Veterinarija Ir Zootechnika"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.11.032"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2010.01603.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg456"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.032730"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.11.034"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.06.009"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.11.028"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.10.007"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.09-082N"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00685.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2010.01096.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.06.016"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.12.009"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01297-9"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00785.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.02.012"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.02.008"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.10.001"},{"issue":"167","key":"e_1_2_9_42_1","first-page":"36","article-title":"Influ\u00eancia da qualidade do s\u00eamen bovino congelado sobre a fertilidade","volume":"28","author":"Severo N. C.","year":"2009","journal-title":"A Hora Veterin\u00e1ria"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.09.026"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.07.022"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.12.015"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/436381"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00343.x"},{"key":"e_1_2_9_48_1","volume-title":"Sperm motility subpopulations and fertility","author":"Van Der Horst G.","year":"2014"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950871"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.03.012"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD17432"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13955","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.13955","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13955","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T20:24:37Z","timestamp":1693340677000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13955"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,29]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/rda.13955"],"URL":"https:\/\/doi.org\/10.1111\/rda.13955","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,29]]},"assertion":[{"value":"2021-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}