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This study investigated the effect of heat shock during in vitro maturation (IVM) on the abundance of bta\u2010miR\u201020a, \u221227b, \u2212103, \u221221\u20105p, \u221219b, \u22121246 miRNAs and <jats:italic>DROSHA<\/jats:italic> and <jats:italic>DICER1<\/jats:italic> mRNAs, previously reported for being involved in oocyte maturation, response to heat stress and miRNA biogenesis. Oocytes were exposed for 12h to heat shock during IVM, fertilized in vitro and the presumptive zygotes cultured for eight days. The relative quantification of miRNAs and mRNAs was performed by real\u2010time PCR in vitro\u2010matured oocytes and 8\u2010cell stage embryos. Progression of meiosis, embryonic development and apoptotic indices was also evaluated. Heat shock compromised (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) oocyte nuclear maturation, cleavage and embryo development, with a higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) embryonic apoptotic index than the control group. The abundance of bta\u2010miR\u201019b increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) whereas the abundance of <jats:italic>DROSHA<\/jats:italic> transcripts decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in embryos derived from heat\u2010shocked oocytes. In conclusion, heat shock during IVM influences the abundance of bta\u2010miR\u201019b and <jats:italic>DROSHA<\/jats:italic> in pre\u2010implantation embryos, indicating a persistent effect of heat shock that can be associated with impaired embryo development.<\/jats:p>","DOI":"10.1111\/rda.13956","type":"journal-article","created":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T11:27:01Z","timestamp":1621682821000},"page":"1128-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Heat shock during in vitro maturation of bovine oocytes disturbs bta\u2010miR\u201019b and DROSHA transcripts abundance after in vitro fertilization"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-1651","authenticated-orcid":false,"given":"Vanessa das Gra\u00e7as Pereira de","family":"Souza","sequence":"first","affiliation":[{"name":"Reproduction and Biotechnology Laboratory Brazilian 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